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  4. Bovine Serum amyloid A,SAA ELISA Kit

Bovine Serum amyloid A,SAA ELISA Kit

  • 中文名稱：
    牛血清淀粉樣蛋白A(SAA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08592b
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛血清淀粉樣蛋白A(SAA)酶聯(lián)免疫試劑盒（CSB-E08592b）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SAA1含量。SAA1即血清淀粉樣蛋白 A1，它在機(jī)體天然免疫中發(fā)揮重要作用，炎癥狀態(tài)下其水平會(huì)顯著升高。研究機(jī)制方面，它涉及多種細(xì)胞信號(hào)通路，與炎癥反應(yīng)、脂質(zhì)代謝等密切相關(guān)，還可能是某些疾病的生物標(biāo)志物或潛在治療靶點(diǎn)。試劑盒檢測范圍為0.1 μg/mL-20 μg/mL，適用于畜牧獸醫(yī)研究、動(dòng)物疾病模型構(gòu)建等領(lǐng)域，可用于探究炎癥相關(guān)疾病的分子機(jī)制、評(píng)估疫苗或藥物的免疫調(diào)節(jié)效果，以及監(jiān)測飼養(yǎng)環(huán)境中牛群健康狀態(tài)，為科研人員提供穩(wěn)定可靠的實(shí)驗(yàn)數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    SAA1 ELISA Kit; SAA ELISA Kit; Serum amyloid A protein ELISA Kit; SAA) [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.1 μg/mL-20 μg/mL
  • 靈敏度：
    0.05 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-96  
    1:2 Average % 93  
    Range % 89-98  
    1:4 Average % 95  
    Range % 91-98  
    1:8 Average % 92  
    Range % 90-95  
  • 回收率：
    The recovery of bovine SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-99  
    EDTA plasma (n=4) 96 89-101  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    0 1.963 1.977 1.970    
    0.1 1.644 1.654 1.649    
    0.4 1.239 1.245 1.242    
    1.6 0.817 0.798 0.808    
    5 0.372 0.368 0.370    
    20 0.121 0.123 0.122    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Major acute phase reactant. Apolipoprotein of the HDL complex.
  • 基因功能參考文獻(xiàn)：
    1. polymorphisms in the SAA2 gene are associated with milk production traits in Chinese Holstein cows PMID: 26373797
  • 相關(guān)疾?。?/div>
    Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    SAA family
  • 組織特異性：
    Expressed by the liver; secreted in plasma.
  • 數(shù)據(jù)庫鏈接：