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  4. Bovine Ubiquitin-like protein ISG15(ISG15) ELISA kit

Bovine Ubiquitin-like protein ISG15(ISG15) ELISA kit

  • 中文名稱：
    牛泛素樣蛋白ISG15(ISG15)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011843BO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛泛素樣蛋白ISG15(ISG15)酶聯(lián)免疫試劑盒（CSB-EL011843BO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ISG15含量。ISG15 是一個(gè)重要靶點(diǎn)。它是一種泛素樣蛋白，在機(jī)體免疫應(yīng)答等方面有重要作用。其研究機(jī)制主要圍繞它與蛋白質(zhì)的結(jié)合修飾，參與調(diào)控免疫信號(hào)通路、抗病毒反應(yīng)等，深入探究 ISG15 有助于揭示疾病發(fā)生發(fā)展機(jī)理并為相關(guān)治療提供思路。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于研究牛類病毒感染機(jī)制（如口蹄疫、牛病毒性腹瀉等）、免疫應(yīng)激反應(yīng)評(píng)估、疫苗開(kāi)發(fā)中的免疫效果監(jiān)測(cè)，以及炎癥相關(guān)疾病模型的分子機(jī)制探索等科研領(lǐng)域，為動(dòng)物免疫學(xué)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    ISG15 ELISA Kit; G1P2 ELISA Kit; ISG17 ELISA Kit; UCRP ELISA Kit; Ubiquitin-like protein ISG15 ELISA Kit; Interferon-stimulated gene product 17 ELISA Kit; Ubiquitin cross-reactive protein ELISA Kit; BoUCRP ELISA Kit
  • 縮寫(xiě)：
    ISG15
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine ISG15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 85-102
    1:2 Average % 92
    Range % 83-99
    1:4 Average % 90
    Range % 85-97
    1:8 Average % 95
    Range % 83-103
  • 回收率：
    The recovery of bovine ISG15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 93 86-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.105 1.995 2.050 1.871
    10 1.654 1.627 1.641 1.462
    5 1.153 1.185 1.169 0.990
    2.5 0.772 0.757 0.765 0.586
    1.25 0.537 0.534 0.536 0.357
    0.625 0.364 0.355 0.360 0.181
    0.312 0.262 0.275 0.269 0.090
    0 0.177 0.181 0.179 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Exhibits antiviral activity towards both DNA and RNA viruses. The secreted form of ISG15 can: induce natural killer cell proliferation, augment lymphokine-activated-killer (LAK) activity, induce dendritic cell maturation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity. The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR which leads to secretion of IFNG and IL10; the interaction is mediated by ITGAL. In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that ISG15 is involved in early blastocyst/embryo development; ISG15 appears to regulate gene expression in developing blastocyst; specifically, ISG15 is required for up-regulation of IFNT expression in blastocyst development. (ISG15 = ubiquitin-like protein ISG15, interferon-stimulated gene 15 protein; IFNT = interferon-tau) PMID: 27165775
    2. ISG 15 gene expression is upregulated during 16-18 days of pregnancy and could be used as an early pregnancy marker in dairy cows, especially in heifers. PMID: 27766692
    3. ISG15, UBE1l and UBCH8 genes are significantly upregulated in the artificially inseminated pregnant cows. PMID: 27802914
    4. Data indicate that the expression profiles of ISG15, MX1, MX2, and OAS1 could be a useful diagnostic biomarker of gestation. PMID: 23384108
    5. Bovine herpesvirus 1 protein bICP0 represses the transcription of bISG15 in fetal bovine lung cells. PMID: 22160940
    6. ISG15 is an antiviral and inducible protein in bovine immunodeficiency virus infected bovin lung cells. PMID: 20569475
    7. ISG15 and conjugated proteins were expressed in corpus luteum of both cyclic and pregnant cows regardless of pregnancy status and were upregulated during early pregnancy PMID: 20172220
    8. interferon-stimulated protein(ISG15) was localized throughout the endometrium on day 18-23 of bovine pregnancy and was specifically localized to organelles and compartments of endometrial epithelial cells and stromal cells PMID: 14563704
    9. rbovISG15 is unstable over time in storage & dialysis. In vivo, conjugation of ISG15 to targeted proteins occurs inside the cell. PMID: 17223698

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  • 亞細(xì)胞定位：
    Cytoplasm. Secreted.
  • 組織特異性：
    Expressed in endometrium and uterine flushings of pregnant cow. Also secreted. Not detected in spleen, liver, corpus luteum or muscle.
  • 數(shù)據(jù)庫(kù)鏈接：