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  4. Bovine Vitamin D-binding protein(GC) ELISA kit

Bovine Vitamin D-binding protein(GC) ELISA kit

  • 中文名稱：
    牛維生素D結(jié)合蛋白(GC)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL009306BO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛維生素D結(jié)合蛋白(GC)酶聯(lián)免疫試劑盒（CSB-EL009306BO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GC含量。試劑盒檢測范圍為0.156 μg/mL-10 μg/mL，適用于科研領(lǐng)域中對牛類動物維生素D代謝通路研究、營養(yǎng)狀態(tài)評估、骨骼發(fā)育相關(guān)機制探索以及免疫調(diào)節(jié)功能分析，可為畜牧營養(yǎng)學(xué)、獸醫(yī)學(xué)及基礎(chǔ)醫(yī)學(xué)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    GCVitamin D-binding protein ELISA kit; DBP ELISA kit; VDB ELISA kit; Gc-globulin ELISA kit; Group-specific component ELISA kit
  • 縮寫：
    GC
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 μg/mL-10 μg/mL
  • 靈敏度：
    0.039 μg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 86  
    Range % 82-92  
    1:200 Average % 93  
    Range % 89-97  
    1:400 Average % 92  
    Range % 84-96  
    1:800 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of bovine GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-96  
    EDTA plasma (n=4) 103 100-106  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.103 0.104 0.104    
    5 0.151 0.155 0.153    
    2.5 0.275 0.292 0.284    
    1.25 0.537 0.540 0.539    
    0.625 0.981 0.923 0.952    
    0.312 1.246 1.328 1.287    
    0.156 1.588 1.724 1.656    
    0 2.424 2.613 2.519    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
  • 基因功能參考文獻：
    1. Associations between SNPs and the studied traits were strongest for SNPs that were located within and immediately upstream of the group-specific component (GC) gene. Our results suggest GC as the gene that underlies the QTL for clinical mastitis and milk production. PMID: 27760518
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 數(shù)據(jù)庫鏈接：