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  4. Bovine adiponectin,ADP ELISA Kit

Bovine adiponectin,ADP ELISA Kit

  • 中文名稱：
    牛脂聯(lián)素(ADP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14054B
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛脂聯(lián)素(ADP)酶聯(lián)免疫試劑盒（CSB-E14054B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADIPOQ含量。ADIPOQ編碼脂聯(lián)素，是脂肪組織分泌的一種蛋白質(zhì)。它具有抗炎、改善胰島素敏感性等作用，與代謝綜合征、心血管疾病等密切相關(guān)。研究機制主要圍繞其信號通路展開，如激活A(yù)MPK等，以調(diào)節(jié)糖脂代謝，為相關(guān)疾病治療帶來新思路。試劑盒檢測范圍為3.125 μg/mL-50 μg/mL，適用于科研領(lǐng)域中對動物代謝調(diào)控機制、營養(yǎng)干預(yù)效果或疾病模型的評估，為肥胖相關(guān)研究、代謝性疾病動物模型構(gòu)建等提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    ADIPOQ ELISA kit; ACRP30 ELISA kit; APM1Adiponectin ELISA kit; 30 kDa adipocyte complement-related protein ELISA kit; Adipocyte complement-related 30 kDa protein ELISA kit; ACRP30 ELISA kit; Adipocyte ELISA kit; C1q and collagen domain-containing protein ELISA kit; Adipose most abundant gene transcript 1 protein ELISA kit; apM-1 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.125 μg/mL-50 μg/mL
  • 靈敏度：
    3.125 μg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-97
    1:2Average %97
    Range %93-104
    1:4Average %85
    Range %82-91
    1:8Average %90
    Range %85-97
  • 回收率：
    The recovery of bovine ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)9894-103
  • 標(biāo)準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    500.212 0.207 0.210
    250.345 0.332 0.339
    12.50.589 0.607 0.598
    6.251.077 1.106 1.092
    3.1252.214 2.123 2.169
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • 基因功能參考文獻：
    1. These data suggest that energy balance around parturition may regulate plasma adiponectin but do not support roles for lipid mobilization or sustained changes in the plasma concentration of leptin, insulin, growth hormone, or fatty acids. PMID: 28843695
    2. Low level expression of adiponectin mRNA was found in all areas of bovine mammary gland tissues examined. AdipoR1 and AdipoR2 mRNAs were also detected in mammary tissues and their expression was particularly prominent in the parenchyma and cistern. PMID: 25676879
    3. Data suggest that genetic variation in promoter region of ADIPOQ (SNPs g.81966235C>T, g.81966377T>C, and g.81966364D>I) contribute to adiposity/marbling in skeletal muscle/meat (and thus meat quality) of Hanwoo beef cattle of North Korea. PMID: 25817801
    4. These data suggest differential contribution of adipose tissue depots to circulating adiponectin. PMID: 24462180
    5. Association analysis indicated that variable duplication within the ADIPOQ gene is associated with body measurements. PMID: 24341634
    6. 14 polymorphisms of the ADIPOQ gene were observed in Chinese cattle; 2 polymorphisms PR_-135 A>G and PR_-68 G>C were located in the core promoter region of ADIPOQ; 3 haplotypes in the 2 polymorphic sites were detected which produce effects on ADIPOQ transcription and are associated with growth traits PMID: 24099391
    7. reduced plasma adiponectin belongs to the subset of hormonal adaptations in EL dairy cows facilitating mammary glucose uptake via promotion of insulin resistance PMID: 23077076
    8. The physiologic status of the ovary has significant effects on the natural expression patterns of adiponectin and its receptors in follicular and luteal cells of bovine ovary. PMID: 20047754
    9. The disulfide bonds help to maintain the mature octadecameric adiponectin structure and stabilize intermediates during the assembly. PMID: 19943704
    10. Gobular adiponectin increased NO production in aortic endothlium by increasing NO synthase activity. PMID: 14551684
    11. A 5bp deletion mutation within the bovine ACRP30 gene was firstly detected and confirmed in 991 cattle . PMID: 18446445
    12. results indicate decreasing adiponectin sensitivity in adipose tissue after calving which might be involved in the reduced insulin sensitivity of adipose tissue during lactation PMID: 19345551
    13. Adiponectin gene was proved to be closely related to carcass and meat quality traits (P < 0.05), which can be used as a candidate molecular marker for production of high-grade meat in Qinchuan beef cattle. PMID: 19840922

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  • 亞細胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：