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  4. Bovine follicle-stimulating hormone(FSH)ELISA Kit

Bovine follicle-stimulating hormone(FSH)ELISA Kit

  • 中文名稱：
    牛促卵泡素(FSH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E15856B
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛促卵泡素(FSH)酶聯(lián)免疫試劑盒（CSB-E15856B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FSH含量。FSH（促卵泡激素）是一種由垂體前葉分泌的激素，主要作用是調(diào)節(jié)性腺活動，如促進女性卵泡生長和男性精子生成。其作用機制是通過與靶細胞表面的FSH受體結(jié)合，引發(fā)細胞內(nèi)信號傳導，進而調(diào)節(jié)相關(guān)基因的表達。FSH在生殖、生長發(fā)育和代謝等方面都發(fā)揮著重要作用。試劑盒檢測范圍為2 mIU/mL-800 mIU/mL，可用于繁殖生物學研究、生殖疾病機制探索、促排卵藥物效果評估；支持從血液循環(huán)系統(tǒng)到性腺組織微環(huán)境的系統(tǒng)性分析，如卵巢卵泡發(fā)育動態(tài)監(jiān)測、睪丸功能評估或垂體 - 性腺軸調(diào)控機制研究；為畜牧繁殖優(yōu)化、生殖內(nèi)分泌學基礎研究及獸藥開發(fā)等科研領(lǐng)域提供精準數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FSHB ELISA Kit; Follitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
  • 縮寫：
    FSH
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    2 mIU/mL-800 mIU/mL
  • 靈敏度：
    2 mIU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-105  
    1:2 Average % 93  
    Range % 89-100  
    1:4 Average % 95  
    Range % 89-100  
    1:8 Average % 97  
    Range % 92-107  
  • 回收率：
    The recovery of bovine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 89-96  
    EDTA plasma (n=4) 94 90-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average    
    800 0.448 0.439 0.444    
    150 0.821 0.824 0.823    
    25 1.354 1.329 1.342    
    7 1.711 1.705 1.708    
    2 2.019 2.011 2.015    
    0 2.555 2.566 2.561    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
  • 基因功能參考文獻：
    1. Study describes generation and characterization of a new Fshb-iCre deleter strain using 4.7 kb of ovine Fshb promoter sequences driving iCre expression exclusively in the gonadotrope lineage with mice developing normally, and fertile. PMID: 26472536
    2. the novel cis-regulatory element generated by the c.-1539_-1538delGGinsTTAACT mutations might play an important role in repressing FSHB transcription, possibly leading to reduced fertility PMID: 26478576
    3. A 200-base region in the bovine FSHbeta 3' untranslated region downregulates reporter expression at the translational level via interactions between AREs in both the 3'UTR and transfactors. PMID: 15189830
    4. The shift in FSH isoforms toward a greater abundance of the less acidic isoforms appears to be a key component in determining the capability for producing a preovulatory rise in estradiol. PMID: 18400447
    5. The effect of a single nucleotide polymorphism of the follicle stimulating hormone beta chain on bull fertility and semen quality is reported. PMID: 18829190

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 數(shù)據(jù)庫鏈接：

    KEGG: bta:281171

    STRING: 9913.ENSBTAP00000014462

    UniGene: Bt.406