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  4. Bovine luteinizing hormone (LH) ELISA kit

Bovine luteinizing hormone (LH) ELISA kit

  • 中文名稱：
    牛黃體生成素（LH）酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12826B
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛黃體生成素（LH）酶聯(lián)免疫試劑盒（CSB-E12826B）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LH含量。LH即黃體生成素，是腺垂體分泌的促性腺激素。其主要作用于性腺，在女性中促進(jìn)排卵和黃體形成，分泌孕激素；在男性中刺激睪丸間質(zhì)細(xì)胞分泌雄激素。研究多聚焦其分泌調(diào)控機(jī)制及在生殖內(nèi)分泌疾病診治中的應(yīng)用。試劑盒檢測(cè)范圍為1.25 mIU/mL-100 mIU/mL，廣泛應(yīng)用于動(dòng)物繁殖生理機(jī)制研究、發(fā)情周期監(jiān)測(cè)、生殖激素調(diào)控實(shí)驗(yàn)等科研領(lǐng)域，亦可用于評(píng)估飼料添加劑或環(huán)境因素對(duì)牛群生殖功能的影響，為畜牧養(yǎng)殖優(yōu)化和基礎(chǔ)醫(yī)學(xué)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    LHBLutropin subunit beta ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit; Lutropin beta chain ELISA kit
  • 縮寫(xiě)：
    LH
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.25 mIU/mL-100 mIU/mL
  • 靈敏度：
    1.56 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 99  
    Range % 95-102  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 88  
    Range % 83-94  
    1:8 Average % 100  
    Range % 95-105  
  • 回收率：
    The recovery of bovine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-95  
    EDTA plasma (n=4) 87 85-92  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average    
    100 0.361 0.368 0.365    
    37.5 0.538 0.567 0.553    
    18.75 0.781 0.765 0.773    
    6.25 1.156 1.126 1.141    
    1.25 1.662 1.698 1.680    
    0 2.161 2.178 2.170    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 數(shù)據(jù)庫(kù)鏈接：