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  4. Bovine neuropeptide Y (NPY) ELISA kit

Bovine neuropeptide Y (NPY) ELISA kit

  • 中文名稱：
    牛神經肽Y(NPY)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08171b
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4800/￥3000
  • 其他：

產品詳情

  • 產品描述：
    牛神經肽Y(NPY)酶聯(lián)免疫試劑盒（CSB-E08171b）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的NPY含量。NPY即神經肽Y，是一種廣泛分布于中樞和外周神經系統(tǒng)的肽類神經遞質。它在調節(jié)攝食行為、能量代謝、心血管活動等方面發(fā)揮重要作用。研究機制上，主要通過與相應受體結合，激活細胞內信號轉導途徑，進而產生一系列生理效應。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產品可用于科研領域探索NPY在牛的能量代謝調控、應激生理響應、神經內分泌網(wǎng)絡中的作用機制，例如在飼喂干預、環(huán)境壓力模型或神經功能研究中動態(tài)監(jiān)測NPY的表達水平變化，為畜牧學、動物生理學及比較醫(yī)學研究提供可靠的工具支持。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    NPY ELISA Kit; Pro-neuropeptide Y [Cleaved into: Neuropeptide Y ELISA Kit; Neuropeptide tyrosine ELISA Kit; NPY); C-flanking peptide of NPY ELISA Kit; CPON)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %87-93
    1:2Average %102
    Range %98-105
    1:4Average %90
    Range %86-96
    1:8Average %91
    Range %88-97
  • 回收率：
    The recovery of bovine NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-94
    EDTA plasma (n=4)9187-95
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.604 2.490 2.547 2.444
    102.113 2.221 2.167 2.064
    51.657 1.711 1.684 1.581
    2.51.114 1.056 1.085 0.982
    1.250.712 0.687 0.700 0.597
    0.6250.413 0.428 0.421 0.318
    0.3120.278 0.260 0.269 0.166
    00.105 0.101 0.103
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
  • 基因功能參考文獻：
    1. Hormonal gene expression involved in residual feed intake in dairy cows may be related to the molecular regulation of the leptin-NPY and insulin signaling pathways. PMID: 26231801
    2. Data indicate that increased body weight gain during juvenile development accelerates sexual maturation in heifers, coincident with reciprocal changes in circulating concentrations of leptin and hypothalamic neuropeptide Y (NPY) release. PMID: 25326602
    3. There is a strong association between putative favorable allelic variants (SNP) of neuropeptide Y, leptin, and IGF-1 genes, and residual feed intake, when animals were grazing on a high-quality, high-availability pasture. PMID: 23881687
    4. The NPY SNP (NPY1) was associated with the prevalence of the animal being in calf 100 d after calving and 305-d milk yield in the first lactation. PMID: 21700051
    5. NPY tended to increase serum growth hormone, which appeared to be a consequence of increased pulse amplitude. Infusion of NPY also increased CSF growth hormone-releasing hormone. PMID: 19632078
    6. The effect of single nucleotide polymorphisms in 6 genes and their associations with production factors in beef cattle are reported. PMID: 17785604
    7. Data show that PKA and PKC pathways are involved in the differential regulation of production of the neuropeptides (Met)enkephalin, galanin, somatostatin, NPY, and VIP. PMID: 18619673

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  • 亞細胞定位：
    Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
  • 蛋白家族：
    NPY family
  • 數(shù)據(jù)庫鏈接：