Bovine pulmonary surfactant-associated protein B,SP-B ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

牛肺表面活性物質(zhì)相關(guān)蛋白B(SP-B)酶聯(lián)免疫試劑盒（CSB-E13834B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFTPB含量。試劑盒檢測范圍為9.37 ng/mL-600 ng/mL，適用于研究SP - B在肺部生理功能、病理機(jī)制（如急性肺損傷或發(fā)育異常）中的表達(dá)調(diào)控，也可用于評估動(dòng)物模型中藥物干預(yù)或基因編輯對SP - B水平的影響。其兼容多種樣本類型的特點(diǎn)，為科研人員在基礎(chǔ)研究和轉(zhuǎn)化醫(yī)學(xué)領(lǐng)域探索呼吸系統(tǒng)相關(guān)分子機(jī)制提供了高效、可靠的分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

SFTPB ELISA Kit; SFTP3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; 6 kDa protein ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
縮寫：

SFTPB
Uniprot No.：

P15781
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

9.37 ng/mL-600 ng/mL
靈敏度：

2.34 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	91
	Range %	85-97
1:4	Average %	90
	Range %	88-94
1:8	Average %	100
	Range %	96-105

回收率：

The recovery of bovine SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	106	103-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
基因功能參考文獻(xiàn)：
1. Measurements of the depth of SP-B(CTERM) indicated the peptide center positions ~8A more deeply than the phosphate headgroups, a topology that may allow the peptide to promote functional lipid structures without causing micellization upon compression. PMID: 22252270
2. results suggest the hydrophobic surfactant protein, SP-B, protects alveolar surfactant phospholiopids from hydrolysis mediated by multiple sPLA(2) in both vesicles (alveolar subphase) and monomolecular films (air-liquid interface) PMID: 22037357
3. The hydrophobic surfactant proteins SP-B and SP-C induce formation of bicontinuous inverse cubic phases. PMID: 20409474
4. Reactive oxygen species inactivation of lipid extract surfactant B (SP-B) biophysical activity appears to involve modification of a maximum of three amino acids: Trp9, Met29, and Met65. PMID: 17425286
亞細(xì)胞定位：

Secreted, extracellular space, surface film.
數(shù)據(jù)庫鏈接：

KEGG: bta:507398

STRING: 9913.ENSBTAP00000042549

UniGene: Bt.42696