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  4. Bovine pulmonary surfactant-associated protein C,SP-C ELISA Kit

Bovine pulmonary surfactant-associated protein C,SP-C ELISA Kit

  • 中文名稱：
    牛肺表面活性物質(zhì)相關(guān)蛋白C(SP-C)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13835B
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛肺表面活性物質(zhì)相關(guān)蛋白C(SP-C)酶聯(lián)免疫試劑盒（CSB-E13835B）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的SFTPC含量。SFTPC編碼的蛋白質(zhì)是肺表面活性物質(zhì)的組成部分，對(duì)維持肺部功能至關(guān)重要。其突變與多種肺部疾病相關(guān)，如間質(zhì)性肺疾病。研究機(jī)制主要圍繞突變?nèi)绾斡绊懙鞍捉Y(jié)構(gòu)和功能、干擾表面活性物質(zhì)穩(wěn)態(tài)，進(jìn)而引發(fā)肺部病變展開(kāi)。試劑盒檢測(cè)范圍為12.5 ng/mL-800 ng/mL，本產(chǎn)品適用于科研領(lǐng)域探索牛肺部疾病的分子機(jī)制、評(píng)估肺損傷模型的生物標(biāo)志物變化，或用于農(nóng)業(yè)研究中監(jiān)測(cè)反芻動(dòng)物肺部健康狀態(tài)。為畜牧學(xué)、獸醫(yī)學(xué)及基礎(chǔ)醫(yī)學(xué)研究提供高效檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    SFTPC ELISA Kit; SFTP2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    12.5 ng/mL-800 ng/mL
  • 靈敏度：
    3.12 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %96
    Range %93-99
    1:2Average %110
    Range %107-113
    1:4Average %95
    Range %91-99
    1:8Average %90
    Range %87-93
  • 回收率：
    The recovery of bovine SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-96
    EDTA plasma (n=4)106101-110
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8002.197 2.168 2.183 2.028
    4001.705 1.732 1.719 1.564
    2001.192 1.168 1.180 1.025
    1000.658 0.689 0.674 0.519
    500.451 0.468 0.460 0.305
    250.367 0.368 0.368 0.213
    12.50.268 0.251 0.260 0.105
    00.154 0.156 0.155
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • 基因功能參考文獻(xiàn)：
    1. The hydrophobic surfactant proteins SP-B and SP-C induce formation of bicontinuous inverse cubic phases. PMID: 20409474
  • 亞細(xì)胞定位：
    Secreted, extracellular space, surface film.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: bta:282071

    STRING: 9913.ENSBTAP00000016672

    UniGene: Bt.8126