Canine C-Reactive Protein,CRP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬C反應(yīng)蛋白(CRP)酶聯(lián)免疫試劑盒（CSB-E14262c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的CRP含量。CRP即C反應(yīng)蛋白，是一種急性時相反應(yīng)蛋白。其水平升高與體內(nèi)炎癥反應(yīng)和組織損傷密切相關(guān)。研究機制上，它能激活補體系統(tǒng)、調(diào)節(jié)免疫細(xì)胞功能等。在臨床中常作為炎癥和疾病活動的重要標(biāo)志物，廣泛用于疾病的診斷和病情監(jiān)測。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL，該產(chǎn)品適用于科研領(lǐng)域中對犬類炎癥相關(guān)疾病模型的機制研究、免疫調(diào)控實驗的效應(yīng)評估，以及藥物或疫苗開發(fā)中炎癥指標(biāo)的動態(tài)監(jiān)測，為實驗動物健康狀態(tài)分析提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CRP
Uniprot No.：

T2KEN6
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

0.312 μg/mL-20 μg/mL
靈敏度：

0.078 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-103
1:2	Average %	89
	Range %	84-96
1:4	Average %	97
	Range %	90-108
1:8	Average %	89
	Range %	84-95

回收率：

The recovery of canine CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	92	87-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.