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  4. Canine Luteinizing Hormone,LH ELISA Kit

Canine Luteinizing Hormone,LH ELISA Kit

  • 中文名稱：
    犬黃體生成素(LH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06790c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4600/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬黃體生成素(LH)酶聯(lián)免疫試劑盒（CSB-E06790c）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LH含量。LH即促黃體生成素，是腺垂體分泌的糖蛋白類(lèi)促性腺激素。它在生殖系統(tǒng)中至關(guān)重要，參與調(diào)節(jié)女性月經(jīng)周期和排卵、男性睪酮合成。研究機(jī)制主要圍繞其與受體結(jié)合，激活下游信號(hào)通路，影響性腺功能和生殖內(nèi)分泌穩(wěn)態(tài)。試劑盒檢測(cè)范圍為0.75 mIU/mL-60 mIU/mL，適用于生殖內(nèi)分泌研究、繁殖障礙機(jī)制探索、絕育手術(shù)前后激素水平追蹤以及繁育管理中的排卵期監(jiān)測(cè)等科研場(chǎng)景，為動(dòng)物生殖生物學(xué)、獸醫(yī)學(xué)基礎(chǔ)研究及寵物繁育技術(shù)開(kāi)發(fā)提供高效檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    LHBLutropin subunit beta ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit; Lutropin beta chain ELISA Kit; Fragment ELISA Kit
  • 縮寫(xiě)：
    LH
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.75 mIU/mL-60 mIU/mL
  • 靈敏度：
    0.94 mIU/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 96  
    Range % 92-99  
    1:4 Average % 101  
    Range % 98-105  
    1:8 Average % 83  
    Range % 80-87  
  • 回收率：
    The recovery of canine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 86 84-92  
    EDTA plasma (n=4) 98 92-104  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average    
    60 0.350 0.357 0.354    
    22.5 0.476 0.468 0.472    
    11.25 0.619 0.605 0.612    
    3.75 1.008 0.998 1.003    
    0.75 1.594 1.512 1.553    
    0 2.008 2.113 2.061    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 數(shù)據(jù)庫(kù)鏈接：