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  4. Canine Matrix metalloproteinase 1,MMP-1 ELISA kit

Canine Matrix metalloproteinase 1,MMP-1 ELISA kit

  • 中文名稱：
    犬基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E14057c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒（CSB-E14057c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MMP-1含量。MMP-1，又稱間質(zhì)膠原酶和成纖維細(xì)胞膠原酶，是基質(zhì)金屬蛋白酶家族的一員，主要參與細(xì)胞外基質(zhì)的降解。其在正常生理過(guò)程中如胚胎發(fā)育和組織重塑中起作用，同時(shí)在疾病過(guò)程中如癌癥和骨關(guān)節(jié)炎中，MMP-1的表達(dá)和活性增加，導(dǎo)致細(xì)胞外基質(zhì)破壞，促進(jìn)疾病進(jìn)展。試劑盒檢測(cè)范圍為0.18 ng/mL-12 ng/mL，適用于科研場(chǎng)景中探究犬類(lèi)疾病模型（如關(guān)節(jié)炎、皮膚損傷修復(fù)或腫瘤微環(huán)境）中MMP-1的動(dòng)態(tài)變化?？蔀榉肿訖C(jī)制研究、藥物開(kāi)發(fā)或生物標(biāo)志物篩選提供定量數(shù)據(jù)支持，滿足科研工作者對(duì)犬類(lèi)MMP-1活性監(jiān)測(cè)的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    MMP-1
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.18 ng/mL-12 ng/mL
  • 靈敏度：
    0.04 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %85-102
    1:2Average %89
    Range %85-99
    1:4Average %94
    Range %85-97
    1:8Average %90
    Range %83-98
  • 回收率：
    The recovery of canine MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9482-98
    EDTA plasma (n=4)9789-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    122.309 2.326 2.318 2.114
    61.754 1.699 1.727 1.523
    31.301 1.338 1.320 1.116
    1.50.931 0.920 0.926 0.722
    0.750.573 0.585 0.579 0.375
    0.370.412 0.407 0.410 0.206
    0.180.299 0.303 0.301 0.097
    00.201 0.206 0.204
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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