Canine Matrix metalloproteinase 13,MMP-13 ELISA kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬基質(zhì)金屬蛋白酶13(MMP-13)酶聯(lián)免疫試劑盒（CSB-E14055c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MMP-13含量。MMP-13即基質(zhì)金屬蛋白酶13，在人體組織重塑、炎癥反應等生理過程中起重要作用。異常表達與多種病理情況相關(guān)，如關(guān)節(jié)炎、癌癥等。研究主要圍繞其酶活性調(diào)控機制，旨在開發(fā)針對此靶點的藥物，以干預相關(guān)疾病進展。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研領域?qū)θ惞顷P(guān)節(jié)疾病模型、軟骨退行性病變機制以及抗炎藥物研發(fā)等研究方向的樣本檢測需求；滿足實驗室對犬MMP - 13蛋白表達水平的高通量檢測及動態(tài)監(jiān)測要求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MMP-13
Uniprot No.：

F1PVS9
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	99
	Range %	95-106
1:4	Average %	87
	Range %	84-92
1:8	Average %	91
	Range %	85-98

回收率：

The recovery of canine MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	88	85-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.