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  4. Canine S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

Canine S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

  • 中文名稱：
    犬S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17221c
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬S100鈣結(jié)合蛋白A12/鈣粒蛋白C(S100A12)酶聯(lián)免疫試劑盒（CSB-E17221c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的S100A12含量。S100A12是一個靶點(diǎn)。它屬于S100蛋白家族，在炎癥反應(yīng)等生理病理過程中起重要作用。研究機(jī)制方面，其可與細(xì)胞表面受體結(jié)合，激活多條信號通路，參與免疫調(diào)節(jié)、細(xì)胞增殖和遷移等過程，是疾病診斷和治療研究的潛在靶點(diǎn)。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品適用于實(shí)驗(yàn)動物模型中炎癥相關(guān)疾病的機(jī)制研究、藥物干預(yù)效果評估以及細(xì)胞實(shí)驗(yàn)中蛋白表達(dá)水平動態(tài)監(jiān)測，其多類型樣本兼容性為組織微環(huán)境分析、體外細(xì)胞模型研究及體液生物標(biāo)志物探索提供可靠工具。為科研工作者解析S100A12在犬類疾病模型中的功能機(jī)制提供高效檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    S100A12
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine S100A12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %81-89
    1:2Average %96
    Range %93-99
    1:4Average %87
    Range %82-91
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of canine S100A12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)9790-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    401.928 2.035 1.982 1.853
    201.460 1.568 1.514 1.385
    100.949 0.928 0.939 0.810
    50.565 0.578 0.572 0.443
    2.50.377 0.398 0.388 0.259
    1.250.252 0.264 0.258 0.129
    0.6250.196 0.198 0.197 0.068
    00.132 0.126 0.129
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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