Canine cardiac isoform of Tropnin T,cTnT ELISA Kit

產(chǎn)品詳情

說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬心肌特異性肌鈣蛋白T(cTnT)酶聯(lián)免疫試劑盒（CSB-E14377c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的cTnT含量。cTnT即心肌肌鈣蛋白T，在心肌損傷檢測(cè)方面意義重大。正常時(shí)血液中含量極低，心肌受損時(shí)釋放入血。研究機(jī)制主要通過檢測(cè)其在血液中的含量變化，來(lái)評(píng)估心肌損傷的程度和范圍，是診斷急性心肌梗死等疾病的關(guān)鍵指標(biāo)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，在心血管疾病機(jī)制研究、心臟毒性評(píng)估及動(dòng)物模型中具有重要科研價(jià)值；適用于探索犬類心臟疾病模型、藥物干預(yù)對(duì)心肌的影響或心肌相關(guān)分子機(jī)制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
縮寫：

cTnT
Uniprot No.：

J9P6P7
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine cTnT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	85-105
1:2	Average %	95
	Range %	85-103
1:4	Average %	94
	Range %	82-100
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of canine cTnT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	82-104
EDTA plasma (n=4)	92	85-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.