Canine connective tissue growth factor,CTGF ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬結締組織生長因子(CTGF)酶聯(lián)免疫試劑盒（CSB-E13386c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CTGF含量。CTGF即結締組織生長因子，是轉化生長因子-β下游的促纖維化介質。它在多種纖維化疾病發(fā)生發(fā)展中起關鍵作用。研究機制上，其能促進細胞黏附、增殖、遷移和細胞外基質合成等，所以是抗纖維化治療極具潛力的靶點。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于犬類纖維化疾病模型研究、器官損傷修復機制探索以及相關代謝性疾病的分子機制分析等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CTGF
Uniprot No.：

J9NTX8
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	93-101
1:4	Average %	85
	Range %	81-89
1:8	Average %	92
	Range %	85-97

回收率：

The recovery of canine CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	108	103-111

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.