Canine follicle-stimulating hormone,FSH ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬促卵泡素(FSH)酶聯(lián)免疫試劑盒（CSB-E06868c）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FSH含量。FSH即促卵泡生成素，是腺垂體分泌的糖蛋白激素，對生殖系統(tǒng)發(fā)育和功能維持至關(guān)重要。其研究機(jī)制主要圍繞調(diào)節(jié)卵泡發(fā)育、精子生成等生殖過程，還涉及與下丘腦-垂體-性腺軸的相互作用，對不孕不育等疾病的治療有重要意義。試劑盒檢測范圍為0.35 mIU/mL-140 mIU/mL，科研人員可將其應(yīng)用于犬類生殖生理學(xué)研究、繁殖障礙機(jī)制探索、生殖相關(guān)藥物或營養(yǎng)干預(yù)效果評估等領(lǐng)域，例如通過檢測發(fā)情周期、卵巢功能異?；虿G丸發(fā)育相關(guān)樣本中的FSH動態(tài)變化，為犬科動物繁殖生物學(xué)研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

FSH
Uniprot No.：

E2RJZ8
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.35 mIU/mL-140 mIU/mL
靈敏度：

0.35 mIU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-106
1:2	Average %	95
	Range %	90-105
1:4	Average %	98
	Range %	93-104
1:8	Average %	92
	Range %	85-99

回收率：

The recovery of canine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	96	92-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.