Canine prealbumin,PA ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬前白蛋白(PA)酶聯(lián)免疫試劑盒（CSB-E13252c）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PA含量。PA（靶點）是具有重要研究價值的靶點。在它可能與特定疾病相關(guān)，成為藥物研發(fā)關(guān)注焦點。研究機制上，科研人員或通過解析其結(jié)構(gòu)、功能及與其他分子相互作用，探尋調(diào)節(jié)途徑，為開發(fā)針對性藥物、治療相應(yīng)疾病奠定基礎(chǔ)。試劑盒檢測范圍為0.625 μg/mL-10 μg/mL，適用于犬類肝臟功能研究、營養(yǎng)干預(yù)實驗、疾病模型構(gòu)建等科研領(lǐng)域，尤其適合探究慢性肝病、代謝綜合征或藥物毒性對蛋白質(zhì)合成的影響本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PA
Uniprot No.：

E2R5U8
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 μg/mL-10 μg/mL
靈敏度：

0.156 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-98
1:200	Average %	103
	Range %	99-106
1:400	Average %	98
	Range %	93-104
1:800	Average %	92
	Range %	87-96

回收率：

The recovery of canine PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-105
EDTA plasma (n=4)	89	84-95

標(biāo)準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.