Chicken Cluster of differentiation 8,CD8ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞CD8分子(CD8)酶聯(lián)免疫試劑盒（CSB-E14317C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD8含量。CD8是重要的免疫相關(guān)靶點(diǎn)。CD8陽性T細(xì)胞為細(xì)胞毒性T細(xì)胞，在機(jī)體免疫防御中發(fā)揮關(guān)鍵作用。其研究機(jī)制主要聚焦于增強(qiáng)CD8 T細(xì)胞的識別和殺傷能力，如通過免疫檢查點(diǎn)抑制劑等，以更好地對抗腫瘤、病毒感染等疾病。試劑盒檢測范圍為3.125 U/mL-800 U/mL，適用于家禽免疫學(xué)基礎(chǔ)研究，如感染性疾病模型中的T細(xì)胞功能分析、疫苗免疫效果評估或免疫調(diào)節(jié)機(jī)制探索等領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CD8
Uniprot No.：

Q6QR64
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.125 U/mL-800 U/mL
靈敏度：

3.125 U/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken CD8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-102
1:2	Average %	89
	Range %	82-94
1:4	Average %	97
	Range %	90-101
1:8	Average %	101
	Range %	94-106

回收率：

The recovery of chicken CD8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	92-108
EDTA plasma (n=4)	92	85-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.