Chicken Glutathione Peroxidase 8 (GPX-8) ELISA Kit
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中文名稱:雞谷胱甘肽過(guò)氧化物酶8(GPX-8)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E14942C
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規(guī)格:96T/48T
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價(jià)格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:雞谷胱甘肽過(guò)氧化物酶8(GPX-8)酶聯(lián)免疫試劑盒(CSB-E14942C)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的GPX-8含量。雞谷胱甘肽過(guò)氧化物酶8(GPX-8)是一種位于內(nèi)質(zhì)網(wǎng)的非特異性抗氧化酶,參與清除活性氧,保護(hù)細(xì)胞膜免受氧化損傷。其基因突變與腫瘤發(fā)生密切相關(guān),在多種腫瘤組織中異常表達(dá),影響腫瘤免疫微環(huán)境和侵襲轉(zhuǎn)移。試劑盒檢測(cè)范圍為31.25 mU/mL-2000 mU/mL,該產(chǎn)品可廣泛應(yīng)用于家禽氧化應(yīng)激機(jī)制研究、營(yíng)養(yǎng)代謝評(píng)估、毒理學(xué)實(shí)驗(yàn)及疾病模型建立等科研領(lǐng)域,例如評(píng)估飼料抗氧化劑效果、監(jiān)測(cè)環(huán)境毒素暴露對(duì)家禽抗氧化系統(tǒng)的影響,或探究傳染性疾病進(jìn)程中GPX - 8的動(dòng)態(tài)變化規(guī)律,為禽類健康管理和基礎(chǔ)醫(yī)學(xué)研究提供可靠的技術(shù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě):GPX-8
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Uniprot No.:
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種屬:Gallus gallus (Chicken)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:31.25 mU/mL-2000 mU/mL
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靈敏度:7.8 mU/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Metabolism
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測(cè)定原理:quantitative
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測(cè)定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of chicken GPX-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 98 Range % 94-103 1:200 Average % 86 Range % 83-90 1:400 Average % 104 Range % 100-108 1:800 Average % 89 Range % 85-93 -
回收率:
The recovery of chicken GPX-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 107 103-110 EDTA plasma (n=4) 84 80-88 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. mU/ml OD1 OD2 Average 2000 0.111 0.115 0.113 1000 0.208 0.210 0.209 500 0.373 0.390 0.382 250 0.642 0.638 0.640 125 0.956 0.961 0.959 62.5 1.146 1.200 1.173 31.25 1.417 1.431 1.424 0 1.634 1.645 1.640 -
數(shù)據(jù)處理:
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貨期:3-5 working days
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