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  4. Chicken Heat Shock Protein 70,Hsp-70 ELISA Kit

Chicken Heat Shock Protein 70,Hsp-70 ELISA Kit

  • 中文名稱：
    雞熱休克蛋白70(HSP-70)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11196Ch
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞熱休克蛋白70(HSP-70)酶聯(lián)免疫試劑盒（CSB-E11196Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HSP70含量。HSP70是一種重要的熱休克蛋白。其背景在于它在細胞應(yīng)激保護中發(fā)揮關(guān)鍵作用，廣泛存在于各類細胞。研究機制方面，它能幫助蛋白質(zhì)正確折疊、防止蛋白聚集，還參與細胞內(nèi)的多種信號轉(zhuǎn)導(dǎo)途徑，在腫瘤、神經(jīng)退行性疾病等研究中備受關(guān)注。試劑盒檢測范圍為0.25 ng/mL-50 ng/mL，其應(yīng)用涵蓋家禽熱應(yīng)激模型評估、疾病相關(guān)蛋白表達調(diào)控研究、免疫應(yīng)答機制探索等科研場景，為禽類生理病理學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Heat shock 70 kDa protein ELISA Kit; HSP70 ELISA Kit
  • 縮寫：
    HSP70
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.25 ng/mL-50 ng/mL
  • 靈敏度：
    0.125 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken Hsp-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 83-97  
    1:2 Average % 91  
    Range % 85-98  
    1:4 Average % 95  
    Range % 91-99  
    1:8 Average % 93  
    Range % 84-99  
  • 回收率：
    The recovery of chicken Hsp-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 92-104  
    EDTA plasma (n=4) 96 90-102  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    0 2.576 2.518 2.547    
    0.25 1.415 1.388 1.402    
    1 1.035 1.047 1.041    
    4 0.610 0.598 0.604    
    12.5 0.329 0.314 0.322    
    50 0.173 0.175 0.174    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release.
  • 基因功能參考文獻：
    1. data indicate that HSP70 is part of the receptor complex of Avian infectious bronchitis virus (IBV) and might help to understand the mechanism of S-mediated cell entry of IBV PMID: 28224252
    2. variation in chicken HSP70 was abundant between and within species PMID: 25867297
    3. The effects of transport time on HSP70 expression, stress, and meat quality are reported. PMID: 25403192
    4. Mn had a dosage-dependent effect on HSP27, HSP40, HSP60, HSP70, and HSP90 mRNA expression in chicken spleen lymphocytes in vitro. PMID: 24081778
    5. Results of this study indicated a long-term enhancement of Hsp70 gene expression associated with improved thermotolerance acquisition in treated chicks without adversely affecting performance. PMID: 23787299
    6. It was concluded that dietary supplementation of Z. officinale and Z. zerumbet powder may induce HSP70 reaction in broiler chickens exposed to heat stress. PMID: 22533311
    7. Data suggest that the expression of HSP70 is heat inducible and tissue dependent and that heat induction may correlate with DNA methylation pattern in the HSP70 promoter. PMID: 23546965
    8. The effects of human interaction on HSP70 expression and stress hormone blood leves in female broiler chickens are reported. PMID: 23243228
    9. It appears that neonatal feed restriction can enhance resistance to Salmonella enteritidis colonization in heat-stressed broiler chicks, and the underlying mechanism could be associated with the lower expression of Hsp70. PMID: 22252346
    10. HSP 70 level increased both in vivo and in vitro trials and may be used as potential molecular and toxicological biomarker. PMID: 19826909
    11. Spinal cord overexpression of Hsp70 enhances motor neuron survival in vivo during the period of naturally occurring cell death. PMID: 16237177
    12. A study evaluating the relationship between tissue or allele and the expression of chicken hsp70 under normal growth conditions and during acute heat stress is reported. PMID: 16905471
    13. The interaction of Meq and Hsp70 is significant in Marek's disease virus oncogenesis. PMID: 19494050
    14. The results suggest that the improved tolerance to transport stress in chicks could be associated with better HSP 70 expression. PMID: 19531704

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  • 蛋白家族：
    Heat shock protein 70 family
  • 數(shù)據(jù)庫鏈接：