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Chicken Immunoglobulin of Yolk,IgY ELISA Kit

  • 中文名稱:
    雞卵黃免疫球蛋白(IgY)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E11635Ch
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    雞卵黃免疫球蛋白(IgY)酶聯(lián)免疫試劑盒(CSB-E11635Ch)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、蛋黃樣本中的IgY含量。IgY即免疫球蛋白Y,是禽類血清和卵黃中的主要抗體。其背景在于可從卵黃高效提取,成本低、產(chǎn)量大。研究機制方面,IgY能特異性結(jié)合抗原,在抗感染、免疫診斷等領(lǐng)域展現(xiàn)作用,如中和病原體、阻斷其與宿主細胞結(jié)合等。試劑盒檢測范圍為0.078 ng/mL-5 ng/mL,靈敏度為0.02 ng/mL。該產(chǎn)品可廣泛應(yīng)用于家禽免疫學(xué)研究、疫苗免疫效果評估、抗體生產(chǎn)工藝優(yōu)化及生物制品質(zhì)量控制等科研場景,為禽類相關(guān)疾病模型的免疫指標分析提供可靠工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    IgY
  • 種屬:
    Gallus gallus (Chicken)
  • 樣本類型:
    serum, plasma, egg yolk
  • 檢測范圍:
    0.078 ng/mL-5 ng/mL
  • 靈敏度:
    0.02 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:500000 Average % 89  
    Range % 81-100  
    1:1000000 Average % 96  
    Range % 87-103  
    1:2000000 Average % 97  
    Range % 92-104  
    1:4000000 Average % 92  
    Range % 86-98  
  • 回收率:
    The recovery of chicken IgY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-105  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    5 2.781 2.911 2.846 2.737  
    2.5 2.316 2.342 2.329 2.220  
    1.25 1.579 1.584 1.582 1.473  
    0.625 0.901 0.883 0.892 0.783  
    0.312 0.443 0.436 0.440 0.331  
    0.156 0.315 0.319 0.317 0.208  
    0.078 0.249 0.247 0.248 0.139  
    0 0.108 0.110 0.109    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 最新研究進展:
    IgY,即鳥類IgY免疫球蛋白,是由鳥類產(chǎn)生的一種特殊類型的免疫球蛋白。最近的研究表明,IgY可以在多個領(lǐng)域中發(fā)揮重要作用,包括食品和醫(yī)藥工業(yè)。例如,IgY被用作生產(chǎn)抗體、免疫增強劑和生物傳感器的材料。此外,IgY還被用作一種潛在的治療腸道疾病的藥物。