Chicken Insulin ELISA kit

產(chǎn)品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞胰島素（Insulin）酶聯(lián)免疫試劑盒（CSB-E13293C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的INS含量。INS即胰島素基因，與血糖調(diào)節(jié)密切相關(guān)。其背景在于胰島素是調(diào)節(jié)血糖的關(guān)鍵激素，INS功能異常會引發(fā)糖尿病等疾病。研究機制主要圍繞基因表達調(diào)控、胰島素合成與分泌過程等，旨在闡明發(fā)病機理，為糖尿病等相關(guān)疾病的治療提供新靶點和策略。試劑盒檢測范圍為3.12 μIU/mL-50 μIU/mL，適用于科研領(lǐng)域家禽代謝調(diào)控機制研究，例如糖尿病模型構(gòu)建、營養(yǎng)代謝相關(guān)疾病探索或藥物干預(yù)后胰島素分泌動態(tài)監(jiān)測等場景，為禽類生理學、病理學及藥理學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
縮寫：

INS
Uniprot No.：

P67970
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 μIU/mL-50 μIU/mL
靈敏度：

0.59 μIU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken insulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	96
	Range %	90-101
1:4	Average %	98
	Range %	95-103
1:8	Average %	90
	Range %	85-96

回收率：

The recovery of chicken insulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	97	95-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
基因功能參考文獻：
1. Data suggest that insulin signaling in skeletal muscle can be regulated by dietary factors (here, dietary content of omega-3 fatty acids). PMID: 24094087
2. Polymorphisms within the INS gene are associated with 55-week body weight. PMID: 16734694
3. The present findings suggest the involvement of insulin in neuronal/glial coupling in the cerebellum. PMID: 19135128
亞細胞定位：

Secreted.
蛋白家族：

Insulin family
數(shù)據(jù)庫鏈接：

KEGG: gga:396145

STRING: 9031.ENSGALP00000010567

UniGene: Gga.673