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  4. Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit

Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit

  • 中文名稱：
    雞胰島素樣生長因子1(IGF-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09867Ch
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞胰島素樣生長因子1(IGF-1)酶聯(lián)免疫試劑盒（CSB-E09867Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IGF1含量。IGF1即胰島素樣生長因子1，在細胞的生長、增殖和分化等過程中扮演重要角色。研究機制方面，它主要通過與IGF1受體結(jié)合，激活下游信號通路，影響細胞的存活、代謝等。其異常表達與多種疾病如腫瘤、糖尿病等密切相關(guān)，是相關(guān)研究的重要靶點。試劑盒檢測范圍為125 pg/mL-2000 pg/mL，該產(chǎn)品可廣泛應(yīng)用于家禽生長發(fā)育機制研究、營養(yǎng)代謝實驗、繁殖生理學(xué)分析以及激素調(diào)控相關(guān)的基礎(chǔ)科研領(lǐng)域，為實驗室提供穩(wěn)定可靠的檢測工具。試劑盒配套包含標準品、檢測板、生物素標記物及顯色試劑等完整組分，操作流程標準化，適用于生命科學(xué)、動物醫(yī)學(xué)等領(lǐng)域的科研實驗需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    125 pg/mL-2000 pg/mL
  • 靈敏度：
    125 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-96  
    1:2 Average % 97  
    Range % 92-105  
    1:4 Average % 87  
    Range % 85-90  
    1:8 Average % 96  
    Range % 93-99  
  • 回收率：
    The recovery of chicken IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-94  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 0.183 0.194 0.189    
    1000 0.311 0.333 0.322    
    500 0.526 0.500 0.513    
    250 1.024 1.028 1.026    
    125 2.107 2.120 2.114    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling.
  • 基因功能參考文獻：
    1. It is a protein synthesis-related factor. PMID: 28594135
    2. The coding region of the IGF1 gene was polymorphic, expressed differentially during the pre-hatch and post-hatch periods, and haplogroups showed significant association with growth traits in chicken. PMID: 26059224
    3. The IGF-I gene is significantly associated with some growth traits (body weight at hatching, and at 4, 8, 12, and 14 weeks of age) in chickens. PMID: 23546986
    4. IGF1 single-nucleotide polymorphism c.47673G > A was associated with body weight and haematocrit percentage, and also with feed intake and percentages of abdominal fat and gizzard genotype x sex interactions. PMID: 23275255
    5. Insulin-like growth factor 1 stimulates Non-astrocytic Inner Retinal Glial cells to proliferate, migrate distally into the retina, and become reactive. PMID: 22973454
    6. The objective of this study was to examine associations of an IGF1 promoter polymorphism with growth, body composition, skeleton integrity, and metabolic traits in chickens. PMID: 15742956
    7. Genetic/nutritional models show a positive relation between muscle IGF-1 mRNA levels, which determine paracrine IGF-1 levels, and post hatch muscle growth. (Review) PMID: 16077194
    8. These results suggest that the mechanism of action whereby IGFBP2 excess impairs long bone development is to inhibit IGF-I and IGF-II mediated proliferation and matrix synthesis. PMID: 16183342
    9. Polymorphisms within the IGF1 gene are associated with 5-week body weight. PMID: 16734694
    10. Blood levels ofIGF-I may be more important than IGF-II after chicken hatching PMID: 17369538
    11. Data show that transcription level of IGF-1 decreased dramatically in the sex-linked dwarf. PMID: 17681929
    12. Association analysis showed that haplotypes based on three cIGF1 polymorphisms (c.-366A>C, c.528G>A and c.*1024C>T) were associated with body weight traits, suggesting that cIGF1 or a tightly linked gene had effects on body weight in the chicken. PMID: 18717967
    13. IGF-1 genes and proteins are expressed at high or moderately high levels in healing tendon PMID: 19084187

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: gga:418090

    STRING: 9031.ENSGALP00000020787

    UniGene: Gga.850