搡老女人多毛老妇女中国,日韩亚洲欧美中文高清在线,人妻少妇一区二区三区,色妞色综合久久夜夜,日本熟妇xxxx

  1. 首頁(yè)
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Chicken Interferon γ,IFN-γ ELISA Kit

Chicken Interferon γ,IFN-γ ELISA Kit

  • 中文名稱(chēng)：
    雞γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08550Ch
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒（CSB-E08550Ch）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IFNG含量。干擾素-γ（IFNG）是一種II型干擾素，主要由自然殺傷細(xì)胞和活化的T細(xì)胞產(chǎn)生，具有抗病毒、免疫調(diào)節(jié)和抗腫瘤特性。其通過(guò)結(jié)合干擾素伽瑪受體（IFNGR）調(diào)節(jié)JAK-STAT通路，激活抗原提呈細(xì)胞，促進(jìn)I型輔助T細(xì)胞（Th1細(xì)胞）的分化，并抑制破骨細(xì)胞形成。研究機(jī)制涉及多種生物過(guò)程和細(xì)胞信號(hào)通路，如蛋白導(dǎo)入細(xì)胞核、轉(zhuǎn)錄調(diào)控、細(xì)胞凋亡和免疫反應(yīng)等。試劑盒檢測(cè)范圍為3.125 pg/mL-800 pg/mL，適用于家禽免疫機(jī)制研究、疫苗效力評(píng)估以及感染性疾病模型中免疫應(yīng)答動(dòng)態(tài)監(jiān)測(cè)，如禽流感、新城疫等病毒性疾病的免疫調(diào)控機(jī)制探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.125 pg/mL-800 pg/mL
  • 靈敏度：
    3.125 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 91-100  
    1:2 Average % 92  
    Range % 88-97  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 94  
    Range % 90-98  
  • 回收率：
    The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 93-101  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    800 0.178 0.182 0.180    
    200 0.245 0.233 0.239    
    50 0.457 0.482 0.470    
    12.5 0.848 0.817 0.833    
    3.125 1.396 1.364 1.380    
    0 2.237 2.242 2.240    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

雞的IFN-gamma試劑盒與其他物種是否有交叉反應(yīng)性？

A:
（雞）與人、大鼠和小鼠重組蛋白無(wú)交叉反應(yīng)性。

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    IFNG，全稱(chēng)為γ-干擾素，是一種由免疫細(xì)胞產(chǎn)生的細(xì)胞因子，在免疫反應(yīng)中起重要作用。最新研究發(fā)現(xiàn)，IFNG在免疫系統(tǒng)中調(diào)節(jié)T細(xì)胞、B細(xì)胞和自然殺傷細(xì)胞等免疫細(xì)胞的功能。IFNG還在感染和炎癥過(guò)程中發(fā)揮重要作用。研究表明，IFNG水平升高與多種疾病有關(guān)，包括自身免疫病、感染性疾病、腫瘤、心血管疾病和神經(jīng)退行性疾病等。近年來(lái)，研究人員正努力探索利用IFNG的治療作用，例如用IFNG治療乳腺癌、肺癌和黑色素瘤等。
  • 功能：
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • 基因功能參考文獻(xiàn)：
    1. Knockdown of IFN-gamma did not seem to fully abrogate vaccine-induced protection against Marek's disease in chickens. PMID: 25673902
    2. Vitiligo in Smyth line chickens appears to be a Th1-polarized autoimmune disease, whereby IFN-gamma expression is strongly associated with parallel increases in IL-10 and IL-21, particularly during the early and active stages of vitiligo. PMID: 22113479
    3. The dose-response effects of supplemental dietary chormium on interferon-gamma expression in response to Newcastle disease virus vaccine in broiler chickens is reported. PMID: 17888472
    4. The pivotal role of chicken interferon-gamma in the pathogenesis and immunosuppression of infectious bursal disease virus infection in in vivo, ex vivo and in vitro experiments are reported. PMID: 17899460
    5. that in vitro stimulation with IBV resulted in ChIFN-gamma production in splenocytes of both infected birds and uninfected birds PMID: 19070879

    顯示更多

    收起更多

  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: gga:396054

    STRING: 9031.ENSGALP00000016086

    UniGene: Gga.916