Chicken Interferon α,IFN-α ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞α干擾素(IFN-α)酶聯(lián)免疫試劑盒（CSB-E10071Ch）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNA1含量。IFNA1即干擾素α1，是I型干擾素家族成員。它具有抗病毒、抗腫瘤和免疫調(diào)節(jié)等多種作用。其研究機制主要是通過與細(xì)胞表面受體結(jié)合，激活下游信號通路，誘導(dǎo)產(chǎn)生多種抗病毒蛋白，抑制病毒復(fù)制和調(diào)節(jié)機體免疫反應(yīng)。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于科研領(lǐng)域?qū)η蓊惷庖呦到y(tǒng)的機制研究；支持科研人員在病毒感染模型構(gòu)建、疫苗免疫效果評估、免疫調(diào)節(jié)劑開發(fā)等研究中動態(tài)監(jiān)測天然免疫應(yīng)答強度；為禽類免疫學(xué)研究提供標(biāo)準(zhǔn)化工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IFNA1; ELISA Kit; IFNA2 ELISA Kit; Interferon type A1/A2 ELISA Kit
縮寫：

IFN-α
Uniprot No.：

P42165
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-95
1:2	Average %	95
	Range %	91-101
1:4	Average %	93
	Range %	86-98
1:8	Average %	95
	Range %	82-98

回收率：

The recovery of chicken IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	96	92-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.