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  4. Chicken Interferon β,IFN-β/IFNB ELISA Kit

Chicken Interferon β,IFN-β/IFNB ELISA Kit

  • 中文名稱：
    雞β干擾素(IFN-β/IFNB)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11237Ch
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞β干擾素(IFN-β/IFNB)酶聯(lián)免疫試劑盒（CSB-E11237Ch）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IFN-β/IFNB含量。IFN-β/IFNB即β-干擾素，是機(jī)體受病毒感染后產(chǎn)生的一類細(xì)胞因子。它能與細(xì)胞表面受體結(jié)合，激活下游信號(hào)通路，誘導(dǎo)抗病毒蛋白產(chǎn)生，抑制病毒復(fù)制和調(diào)節(jié)免疫反應(yīng)，在抗病毒、抗腫瘤及免疫相關(guān)疾病研究中受關(guān)注。試劑盒檢測(cè)范圍為2.5 pg/mL-200 pg/mL，適用于科研場(chǎng)景中禽類免疫機(jī)制研究、疫苗效力評(píng)價(jià)或抗病毒藥物開發(fā)等方向；可應(yīng)用于評(píng)估病毒感染模型、免疫刺激實(shí)驗(yàn)或比較不同組織微環(huán)境中IFN-β的動(dòng)態(tài)表達(dá)水平本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IFNB ELISA Kit; IFN2 ELISA Kit; Interferon type B ELISA Kit; IFN-beta ELISA Kit
  • 縮寫：
    IFN-β/IFNB
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    2.5 pg/mL-200 pg/mL
  • 靈敏度：
    3.125 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 99  
    Range % 95-103  
    1:2 Average % 94  
    Range % 88-98  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 89  
    Range % 84-96  
  • 回收率：
    The recovery of chicken IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 88-94  
    EDTA plasma (n=4) 88 82-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.342 0.341 0.342    
    75 0.494 0.486 0.490    
    37.5 0.652 0.664 0.658    
    12.5 1.067 1.076 1.072    
    2.5 1.478 1.575 1.527 　  
    0 1.854 1.955 1.905    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Has antiviral activities.
  • 基因功能參考文獻(xiàn)：
    1. Fpv012 inhibits induction of IFNB. PMID: 23427153
    2. RNA interference-mediated knockdown of ChMda5 showed that ChMda5 plays an important role in the IFN response of chicken cells to dsRNA PMID: 21444763
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 數(shù)據(jù)庫鏈接：