Chicken Interleukin 12,IL-12/70 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞白介素12(IL-12/70)酶聯(lián)免疫試劑盒（CSB-E12836C）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL12/70含量。IL12/70作為靶點(diǎn)，IL-12和IL-70在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用。針對(duì)它的研究聚焦于通過(guò)阻斷其信號(hào)通路，調(diào)節(jié)免疫細(xì)胞活性及功能來(lái)治療疾病，如自身免疫病、腫瘤等，旨在抑制異常免疫反應(yīng)或增強(qiáng)抗腫瘤免疫。試劑盒檢測(cè)范圍為0.78 pg/mL-200 pg/mL，適用于家禽免疫機(jī)制研究、疫苗效力評(píng)估、病原感染模型中的細(xì)胞因子動(dòng)態(tài)監(jiān)測(cè)等科研場(chǎng)景，為禽類免疫相關(guān)疾病的分子機(jī)制探索提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

IL-12/70
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.78 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-12/70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-98
1:2	Average %	94
	Range %	90-98
1:4	Average %	92
	Range %	87-98
1:8	Average %	96
	Range %	92-101

回收率：

The recovery of chicken IL-12/70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	92	88-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.