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  4. Chicken Interleukin 18,IL-18 ELISA KIT

Chicken Interleukin 18,IL-18 ELISA KIT

  • 中文名稱：
    雞白介素18(IL-18)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E10070Ch
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞白介素18(IL-18)酶聯(lián)免疫試劑盒（CSB-E10070Ch）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL18含量。IL-18，又稱干擾素-γ誘導(dǎo)因子，是一種促炎細(xì)胞因子，屬于IL-1超家族。它主要由巨噬細(xì)胞和其他細(xì)胞產(chǎn)生，能夠誘導(dǎo)T細(xì)胞產(chǎn)生干擾素-γ。IL-18在免疫調(diào)節(jié)中起重要作用，參與先天性和適應(yīng)性免疫反應(yīng)的調(diào)節(jié)。其研究機(jī)制涉及IL-18與IL-18R的結(jié)合，以及通過(guò)激活下游信號(hào)通路來(lái)調(diào)節(jié)免疫細(xì)胞的功能。試劑盒檢測(cè)范圍為20 pg/mL-500 pg/mL，適用于家禽免疫學(xué)研究、疫苗效力評(píng)估或感染性疾病模型的建立?？蒲腥藛T可通過(guò)精準(zhǔn)分析雞類樣本中IL - 18的表達(dá)水平，探究其在傳染性法氏囊病、新城疫等病原體感染過(guò)程中的免疫調(diào)控機(jī)制，或評(píng)估營(yíng)養(yǎng)干預(yù)、環(huán)境應(yīng)激對(duì)家禽免疫功能的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    IL18 ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue Homogenates
  • 檢測(cè)范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-102  
    1:2 Average % 90  
    Range % 82-99  
    1:4 Average % 93  
    Range % 85-102  
    1:8 Average % 96  
    Range % 92-100  
  • 回收率：
    The recovery of chicken IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 81-99  
    EDTA plasma (n=4) 95 89-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    500 1.981 1.927 1.954 1.851  
    250 1.334 1.374 1.354 1.251  
    125 0.920 0.915 0.918 0.815  
    62.5 0.619 0.643 0.631 0.528  
    31.2 0.395 0.376 0.386 0.283  
    15.6 0.290 0.306 0.298 0.195  
    7.8 0.211 0.224 0.218 0.115  
    0 0.102 0.103 0.103    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
  • 亞細(xì)胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫(kù)鏈接：