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  4. Chicken Interleukin 1β,IL-1β ELISA Kit

Chicken Interleukin 1β,IL-1β ELISA Kit

  • 中文名稱：
    雞白介素1β(IL-1β)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11230Ch
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞白介素1β(IL-1β)酶聯(lián)免疫試劑盒（CSB-E11230Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B 即白細(xì)胞介素-1β，是一種重要的促炎細(xì)胞因子。在炎癥反應(yīng)、免疫調(diào)節(jié)等生理和病理過程中發(fā)揮關(guān)鍵作用。研究機(jī)制上，它可激活下游信號通路，引發(fā)炎癥級聯(lián)反應(yīng)，與多種炎癥相關(guān)疾病如關(guān)節(jié)炎、心血管病等發(fā)病密切相關(guān)。試劑盒檢測范圍為0.27 pg/mL-200 pg/mL，特別適用于家禽免疫學(xué)研究、疫苗效力評估、感染性疾病模型建立等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IL-1β
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.27 pg/mL-200 pg/mL
  • 靈敏度：
    0.27 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-100  
    1:2 Average % 99  
    Range % 95-106  
    1:4 Average % 84  
    Range % 80-88  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of chicken IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-98  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.173 0.169 0.171    
    66.7 0.238 0.259 0.249    
    22.2 0.339 0.367 0.353    
    7.4 0.459 0.488 0.474    
    2.5 0.602 0.633 0.618    
    0.82 0.817 0.855 0.836    
    0.27 1.033 1.008 1.021    
    0 1.748 1.672 1.710    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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