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  4. Chicken Interleukin 4,IL-4 ELISA Kit

Chicken Interleukin 4,IL-4 ELISA Kit

  • 中文名稱：
    雞白介素4(IL-4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06756Ch
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E06756Ch）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-4含量。IL-4是重要的免疫調(diào)節(jié)細胞因子，在多種免疫和炎癥反應(yīng)中起關(guān)鍵作用。研究發(fā)現(xiàn)它可促進B細胞增殖分化，調(diào)節(jié)Th2細胞免疫，與哮喘、特應(yīng)性皮炎等疾病的發(fā)生發(fā)展密切相關(guān)。目前圍繞它阻斷信號傳導(dǎo)等機制的研究，有望帶來新治療策略。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于科研領(lǐng)域?qū)﹄u免疫系統(tǒng)功能的研究，如傳染病模型中的細胞因子動態(tài)分析、疫苗免疫效果評估或免疫相關(guān)疾病的分子機制探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    IL-4
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    12.5 pg/mL-800 pg/mL
  • 靈敏度：
    3.12 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %80-95
    1:2Average %94
    Range %88-101
    1:4Average %93
    Range %86-106
    1:8Average %95
    Range %82-100
  • 回收率：
    The recovery of chicken IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-97
    EDTA plasma (n=4)9285-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8001.726 1.825 1.776 1.665
    4001.336 1.383 1.360 1.249
    2001.060 0.981 1.021 0.910
    1000.714 0.706 0.710 0.599
    500.481 0.475 0.478 0.367
    250.307 0.292 0.300 0.189
    12.50.227 0.214 0.221 0.110
    00.108 0.113 0.111 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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