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  4. Chicken Interleukin 8(IL-8) ELISA Kit

Chicken Interleukin 8(IL-8) ELISA Kit

  • 中文名稱：
    雞白介素8(IL-8)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14191C
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞白介素8(IL-8)酶聯(lián)免疫試劑盒（CSB-E14191C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CXCL8含量。CXCL8即趨化因子配體8，是重要的促炎細胞因子。它在炎癥反應(yīng)、腫瘤發(fā)生發(fā)展等過程中扮演關(guān)鍵角色。其研究機制主要圍繞與受體結(jié)合，激活下游信號通路，促進細胞趨化、增殖等，在免疫調(diào)節(jié)和疾病病理進程研究中備受關(guān)注。試劑盒檢測范圍為1.56 pg/mL-400 pg/mL，該產(chǎn)品廣泛用于家禽免疫機制研究、疫苗效力評估、病原感染模型建立等科研領(lǐng)域，特別適用于雞傳染性支氣管炎、新城疫等疾病模型中炎癥因子的動態(tài)監(jiān)測，以及免疫調(diào)節(jié)劑或飼料添加劑對免疫系統(tǒng)影響的量化評估。實驗支持多種樣本類型檢測，組織勻漿檢測功能為深入解析局部組織免疫反應(yīng)提供技術(shù)保障。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CXCL8 ELISA Kit; CEF4 ELISA Kit; EMF1 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; 9E3 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Embryo fibroblast protein 1 ELISA Kit; EMF-1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 pg/mL-400 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 102  
    Range % 97-106  
    1:2 Average % 88  
    Range % 84-93  
    1:4 Average % 104  
    Range % 99-109  
    1:8 Average % 103  
    Range % 99-106  
  • 回收率：
    The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-102  
    EDTA plasma (n=4) 91 85-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.187 0.183 0.185    
    100 0.259 0.267 0.263    
    25 0.471 0.452 0.462    
    6.25 0.862 0.843 0.853    
    1.56 1.410 1.398 1.404    
    0 2.251 2.159 2.205    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

Can I get a copy of the kit instructions to as well as any references to papers that have used this kit? I want to make sure it will work in our specific use case. To that end I'm looking for information about the nature of the antibodies (capture and detection) are they monoclonal or poly. Are they the same Ab for both steps or different? Are the plates recoated, or do we need to coat them ourselves? Thank you for the help.

A:
Thanks for your inquiry.
I will send you the manual by e-mail.
Here is the reference about this kit: https://www.sciencedirect.com/science/article/pii/S0165242717302362
The plate is ready to use and you don't need to coat it by yourself. The plate is coated with specific antigen.There is only one antibody in the kit and it is goat polyclonal antibody.
Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 功能：
    May be an autocrine factor that promotes the growth of fibroblasts and is involved in the neoplastic transformation of fibroblasts by v-Src. Chemotactic for peripheral blood mononuclear cells as well as for heterophils.
  • 基因功能參考文獻：
    1. Lower levels in airway aspirates from breastfed infants with acute bronchiolitis PMID: 20337964
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 數(shù)據(jù)庫鏈接：

    KEGG: gga:396495

    STRING: 9031.ENSGALP00000019051

    UniGene: Gga.826