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  4. Chicken Tumor necrosis factor α,TNF-α ELISA KIT

Chicken Tumor necrosis factor α,TNF-α ELISA KIT

  • 中文名稱：
    雞腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11231Ch
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    雞腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒（CSB-E11231Ch）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TNF-α含量。TNF-α（腫瘤壞死因子-α）是一種多功能促炎細(xì)胞因子，主要在免疫應(yīng)答、炎癥反應(yīng)和細(xì)胞凋亡中發(fā)揮作用。其研究機(jī)制涉及與受體TNFR1結(jié)合，激活多種信號(hào)通路，如線粒體途徑、Bcl-2家族蛋白、活性氧等，導(dǎo)致細(xì)胞凋亡和炎癥反應(yīng)。TNF-α已成為治療自身免疫疾病的關(guān)鍵靶點(diǎn)。試劑盒檢測(cè)范圍為0.27 pg/mL-200 pg/mL，適用于科研領(lǐng)域研究禽類傳染性疾病（如禽流感、新城疫）的炎癥反應(yīng)機(jī)制，評(píng)估疫苗或抗炎藥物對(duì)免疫系統(tǒng)的調(diào)控效果，以及構(gòu)建家禽疾病模型時(shí)監(jiān)測(cè)炎癥因子動(dòng)態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
  • 縮寫：
    TNF-α
  • 種屬：
    Gallus gallus (Chicken)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.27 pg/mL-200 pg/mL
  • 靈敏度：
    0.27 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-96  
    1:2 Average % 100  
    Range % 95-105  
    1:4 Average % 87  
    Range % 82-91  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of chicken TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 93-108  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.173 0.170 0.172    
    66.7 0.259 0.251 0.255    
    22.2 0.347 0.367 0.357    
    7.41 0.452 0.488 0.470    
    2.47 0.611 0.633 0.622    
    0.82 0.809 0.855 0.832    
    0.27 1.033 1.018 1.026    
    0 1.748 1.707 1.728    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    TNF-α（腫瘤壞死因子-α）是一種促炎癥蛋白質(zhì)，它在多種疾病中扮演重要角色。最新研究表明，TNF-α可能參與COVID-19的發(fā)病機(jī)制，因?yàn)檫@種病毒通過激活NF-κB途徑來增加TNF-α的表達(dá)，從而引發(fā)炎癥反應(yīng)和危及生命的并發(fā)癥。此外，TNF-α也參與了許多自身免疫疾病的發(fā)生和發(fā)展過程，例如風(fēng)濕性關(guān)節(jié)炎和腸道炎癥性疾病。