Chicken rudimental bovine serum albumin(BSA) check-up ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

雞的牛血清白蛋白(BSA) 殘留檢測(cè)酶聯(lián)免疫試劑盒（CSB-EQ027303CH）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的BSA含量。試劑盒檢測(cè)范圍為0.312 μg/mL-80 μg/mL，靈敏度為0.312 μg/mL。適用于科研領(lǐng)域中對(duì)生物制品、疫苗生產(chǎn)或?qū)嶒?yàn)樣本處理流程中BSA污染物的質(zhì)量控制；適用于評(píng)估樣本純化工藝效果、細(xì)胞培養(yǎng)基質(zhì)殘留分析或生物制藥流程中的BSA監(jiān)控；助力科研人員優(yōu)化實(shí)驗(yàn)體系并保障數(shù)據(jù)可靠性本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

BSA
種屬：

Gallus gallus (Chicken)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.312 μg/mL-80 μg/mL
靈敏度：

0.312 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	106
	Range %	100-112
1:4	Average %	95
	Range %	90-100
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of chicken BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	92	88-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.