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  4. Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

  • 中文名稱：
    鹿胰島素樣生長(zhǎng)因子1(IGF-1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12644De
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    鹿胰島素樣生長(zhǎng)因子1(IGF-1)酶聯(lián)免疫試劑盒（CSB-E12644De）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IGF-1含量。IGF-1（胰島素樣生長(zhǎng)因子-1）是一種由IGF1基因編碼的蛋白質(zhì)，與胰島素具有相似的結(jié)構(gòu)和功能。它主要在肝臟合成，并通過(guò)生長(zhǎng)激素（GH）的調(diào)節(jié)促進(jìn)生長(zhǎng)和發(fā)育。IGF-1通過(guò)激活I(lǐng)GF-1受體，在多個(gè)組織和器官中調(diào)節(jié)細(xì)胞增殖、分化和凋亡。其研究機(jī)制涉及IGF-1與受體的結(jié)合，以及通過(guò)多種信號(hào)通路（如PI3K/AKT和MEK/ERK）影響細(xì)胞功能。試劑盒檢測(cè)范圍為1 ng/mL-200 ng/mL，科研人員可將其應(yīng)用于鹿科動(dòng)物生長(zhǎng)發(fā)育研究、營(yíng)養(yǎng)干預(yù)實(shí)驗(yàn)、繁殖生物學(xué)分析以及代謝相關(guān)疾病模型的機(jī)制探索，例如評(píng)估不同飼養(yǎng)條件下IGF - 1的動(dòng)態(tài)變化，或探究其在組織再生修復(fù)中的作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 縮寫：
    IGF-1
  • Uniprot No.：
  • 種屬：
    Deer
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1 ng/mL-200 ng/mL
  • 靈敏度：
    0.5 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %98
    Range %92-104
    1:2Average %95
    Range %89-104
    1:4Average %99
    Range %87-107
    1:8Average %96
    Range %90-102
  • 回收率：
    The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9690-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    2000.175 0.174 0.175
    500.333 0.316 0.325
    160.622 0.640 0.631
    41.040 1.048 1.044
    11.416 1.389 1.403
    02.577 2.633 2.605
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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