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  4. Dog Interleukin-8,IL-8 ELISA Kit

Dog Interleukin-8,IL-8 ELISA Kit

  • 中文名稱：
    犬白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08052c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒（CSB-E08052c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CXCL8含量。CXCL8作為一個(gè)靶點(diǎn)，它屬于趨化因子家族，與炎癥反應(yīng)、細(xì)胞遷移等密切相關(guān)。研究發(fā)現(xiàn)，它可以通過(guò)與特定受體結(jié)合，激活下游信號(hào)通路，在腫瘤、自身免疫病等疾病發(fā)展進(jìn)程中發(fā)揮作用，是當(dāng)前藥物研發(fā)等領(lǐng)域的重要研究對(duì)象。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，靈敏度為0.146 ng/mL。本試劑盒通過(guò)高靈敏度抗體組合實(shí)現(xiàn)0.312 ng/mL至20 ng/mL的寬泛檢測(cè)范圍，可精準(zhǔn)量化低豐度目標(biāo)蛋白，滿足科研場(chǎng)景中細(xì)胞因子動(dòng)態(tài)監(jiān)測(cè)、炎癥模型評(píng)估或藥物干預(yù)效果分析等需求。其操作流程兼容常規(guī)實(shí)驗(yàn)室設(shè)備，適用于獸醫(yī)基礎(chǔ)研究、實(shí)驗(yàn)動(dòng)物模型開發(fā)及生物制劑研發(fā)等領(lǐng)域，為探索犬類免疫系統(tǒng)功能及相關(guān)疾病機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.146 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-97  
    1:2 Average % 99  
    Range % 95-105  
    1:4 Average % 88  
    Range % 85-93  
    1:8 Average % 90  
    Range % 86-97  
  • 回收率：
    The recovery of dog IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 86-94  
    EDTA plasma (n=4) 88 85-95  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 3.221 3.295 3.258 3.157  
    10 2.523 2.711 2.617 2.516  
    5 1.791 1.930 1.861 1.760  
    2.5 1.035 1.045 1.040 0.939  
    1.25 0.466 0.485 0.476 0.375  
    0.625 0.249 0.259 0.254 0.153  
    0.312 0.157 0.167 0.162 0.061  
    0 0.103 0.099 0.101    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
    IL8，全稱為白細(xì)胞介素8，是一種由免疫細(xì)胞產(chǎn)生的炎癥介質(zhì)。IL8通過(guò)結(jié)合CXCR1和CXCR2等受體調(diào)節(jié)免疫細(xì)胞的趨化和活化，參與多種炎癥反應(yīng)的發(fā)生和發(fā)展。近年來(lái)的研究表明，IL8在腫瘤的發(fā)生和轉(zhuǎn)移中發(fā)揮重要作用。例如，IL8的高表達(dá)與乳腺癌、結(jié)直腸癌等多種腫瘤的不良預(yù)后相關(guān)。
  • 功能：
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
  • 基因功能參考文獻(xiàn)：
    1. Analysis of ROC suggests the use of serum levels of MCP-1 and IL-7 as predictors of the occurrence of complications with an AUC of 0.906 and 0.896 respectively and linear combinations of MCP-1, KC-Like, IL-7 and GM-CSF with values up to AUC = 0.983 PMID: 29304171
    2. These observations indicate that the individual activation of ERK2 and JNK1 pathways contributes to TNF-alpha-induced IL-8 expression in synovial fibroblasts. PMID: 28806729
    3. data suggest that CCL2 acts as a PMNs chemotactic factor as well as CXCL8, and both CCL2 and CXCL8 facilitate the infiltration of PMNs into the joints of idiopathic polyarthritis PMID: 27863550
    4. The study findings suggest that both CCL2 and CXCL8 are involved in the pathogenesis of canine idiopathic pulmonary fibrosis. PMID: 26231926
    5. High CXCL8 blood concentrations might be related to the breed predisposition of the West Highland white terrier for canine idiopathic pulmonary fibrosis. PMID: 26267090
    6. IL-8-positive macrophages were significantly increased in large polyps compared to controls PMID: 24148828
    7. hemangiosarcoma derived IL-8 may play a role in tumor development by modulating the tumor microenvironment PMID: 24582862
    8. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
    9. serum and tumor levels of IL-8 and IL-10 in dogs bearing benign and malignant mammary tumors, including dogs with inflammatory mammary cancer, for a better understanding of this disease PMID: 23351639
    10. Sixty-one percent (28/46) of the samples from canine stifle osteoarthritis had IL-8 mRNA expression in contrast to 4% (1/24) in the control stifle joints PMID: 16102844
    11. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 數(shù)據(jù)庫(kù)鏈接：