Dog N-terminal Pro atrial natriuretic peptide (NT-ProANP)ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬N端前心鈉肽(NT-ProANP)酶聯(lián)免疫試劑盒（CSB-EQ027254DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的NT-ProANP含量。NT - ProANP是一種重要靶點(diǎn)。它是心房利鈉肽原經(jīng)酶切產(chǎn)生的無活性片段。背景上，其水平變化與心血管疾病密切相關(guān)。研究機(jī)制方面，檢測其濃度可輔助診斷心衰等疾病，能反映心臟功能狀態(tài)，在心血管疾病診治中有重要價(jià)值。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品可應(yīng)用于犬類心血管疾病機(jī)制研究、心臟功能評估模型構(gòu)建，以及藥物干預(yù)或生理病理?xiàng)l件下心臟相關(guān)分子變化的動(dòng)態(tài)監(jiān)測，為動(dòng)物實(shí)驗(yàn)中心臟健康研究提供高效工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

NT-ProANP
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog NT-ProANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	91-105
1:2	Average %	97
	Range %	91-100
1:4	Average %	100
	Range %	92-106
1:8	Average %	95
	Range %	86-98

回收率：

The recovery of dog NT-ProANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	99	95-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.