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  4. Dog Renin ELISA Kit

Dog Renin ELISA Kit

  • 中文名稱：
    犬腎素(Renin)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08704c
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4800/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬腎素(Renin)酶聯(lián)免疫試劑盒（CSB-E08704c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的REN含量。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產(chǎn)品適用于實驗動物模型（如高血壓、腎功能損傷等）中腎素活性變化的動態(tài)監(jiān)測，或用于藥物干預、病理生理機制研究中的分子水平分析，為心血管及代謝性疾病的基礎科研提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    REN ELISA Kit; Renin ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-97  
    1:2 Average % 105  
    Range % 100-112  
    1:4 Average % 84  
    Range % 80-88  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of dog renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 90-103  
    EDTA plasma (n=4) 88 84-93  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    4000 2.582 2.683 2.633 2.454  
    2000 2.204 2.104 2.154 1.975  
    1000 1.451 1.521 1.486 1.307  
    500 0.864 0.908 0.886 0.707  
    250 0.569 0.584 0.577 0.398  
    125 0.430 0.402 0.416 0.237  
    62.5 0.279 0.259 0.269 0.090  
    0 0.182 0.176 0.179    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
  • 基因功能參考文獻：
    1. With a moderate deficit in total body sodium and total body water, the renin-angiotensin-aldosterone system is the major factor controlling arterial blood pressure. PMID: 19930429
    2. Characterization of canine renin with mutations in the substrate binding site. PMID: 17942478
    3. Normotensive sodium loading in conscious dogs: regulation of renin secretion during beta1-receptor blockade. PMID: 19073902
  • 亞細胞定位：
    Secreted. Membrane.
  • 蛋白家族：
    Peptidase A1 family
  • 數(shù)據(jù)庫鏈接：