Dog adrenomedullin,ADM ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬腎上腺髓質(zhì)素(ADM)酶聯(lián)免疫試劑盒（CSB-E09836c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的ADM含量。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，適用于體外實(shí)驗(yàn)環(huán)境下探索ADM在犬類疾病模型中的調(diào)控機(jī)制、藥物干預(yù)研究或基礎(chǔ)生理功能解析，為動(dòng)物醫(yī)學(xué)及比較醫(yī)學(xué)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

ADM ELISA Kit; ADM [Cleaved into: Adrenomedullin ELISA Kit; AM); Proadrenomedullin N-20 terminal peptide ELISA Kit; ProAM N-terminal 20 peptide ELISA Kit; PAMP ELISA Kit; ProAM-N20)] ELISA Kit
縮寫：

ADM
Uniprot No.：

O77559
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates, cell culture supernates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-99
1:2	Average %	96
	Range %	91-104
1:4	Average %	98
	Range %	90-110
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of dog ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-104
EDTA plasma (n=4)	97	90-110

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.