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  4. Dog insulin-like growth factor 1 (IGF1) ELISA kit

Dog insulin-like growth factor 1 (IGF1) ELISA kit

  • 中文名稱：
    犬胰島素樣生長因子1(IGF1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011086DO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬胰島素樣生長因子1(IGF1)酶聯(lián)免疫試劑盒（CSB-EL011086DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IGF1含量。IGF1即胰島素樣生長因子1，在細(xì)胞生長、增殖和分化等生理過程中起關(guān)鍵作用。其背景在于與生長發(fā)育密切相關(guān)。研究機(jī)制方面，主要通過與受體結(jié)合激活下游信號通路，影響細(xì)胞生命活動(dòng)，在腫瘤、代謝疾病等研究中備受關(guān)注。試劑盒檢測范圍為31.25 ng/mL-2000 ng/mL，適用于犬類代謝疾病機(jī)制研究、生長發(fā)育評估、營養(yǎng)干預(yù)實(shí)驗(yàn)以及內(nèi)分泌相關(guān)動(dòng)物模型的數(shù)據(jù)采集，為探索IGF1在犬類生理病理過程中的調(diào)控網(wǎng)絡(luò)提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IGF1 ELISA Kit; IGFIAInsulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 ng/mL-2000 ng/mL
  • 靈敏度：
    7.8 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IGF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 94-102  
    1:2 Average % 92  
    Range % 88-96  
    1:4 Average % 105  
    Range % 100-110  
    1:8 Average % 95  
    Range % 90-99  
  • 回收率：
    The recovery of dog IGF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-100  
    EDTA plasma (n=4) 87 80-91  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    2000 2.544 2.487 2.516 2.351  
    1000 1.964 2.006 1.985 1.820  
    500 1.305 1.394 1.350 1.185  
    250 0.690 0.738 0.714 0.549  
    125 0.448 0.482 0.465 0.300  
    62.5 0.361 0.339 0.350 0.185  
    31.25 0.257 0.245 0.251 0.086  
    0 0.162 0.168 0.165    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
  • 基因功能參考文獻(xiàn)：
    1. This study suggested that serum 26SP concentrations seem to be increased in some dogs suffering from malignant tumors, especially in dogs with mammary carcinoma and smaller tumors and that increased serum IGF-1 concentrations could be an indication of large tumors and a poor prognosis. PMID: 29755191
    2. Leptin and insulin like growth factor 1 may support spermatogenesis in adult dogs and mediate Leydig cell function PMID: 28101891
    3. This study evaluated the role of IGF1 and phosphoinositol 3 kinase signaling in the pathogenesis of adrenocortical tumors in dogs and to identify pathway components that may hold promise as future therapeutic targets or as prognostic markers. PMID: 25619516
    4. These data strongly indicated a strong relathionship between fetal fluids IGF-I and nonesterified fatty acid concentrations and breed size exists. PMID: 24674237
    5. This study found that serum protein profiles and IGF-I concentrations were altered in both diabetic and insulin-treated animals. PMID: 22122898
    6. There was significantly higher expression of genes encoding for growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in metastases compared to their primary tumours. PMID: 21507690
    7. This study focuses on the co-expression of interleukin-4 (IL-4) and insulin-like-growth factor-1 (IGF-1), which specifically target inflammation and cartilage repair, respectively. PMID: 20188584
    8. variability in height between dogs were associated with dramatic differences in insulin-like growth factor 1(IGF1) and IGF binding protein 3 levels in plasma and follicular fluid and differences were associated with functional aspects of ovarian follicles PMID: 20406781
    9. inhibiting GSK3 increases brain insulin-like growth factor I levels PMID: 20351102
    10. a single IGF1 single-nucleotide polymorphism haplotype is common to all small breeds and nearly absent from giant breeds, suggesting that the same causal sequence variant is a major contributor to body size in all small dogs PMID: 17412960
    11. Genetics are associated with body size in dogs. PMID: 17502080
    12. Study provides evidence that progesteron might increase autocrine Growth Hormone production which might directly stimulate local or systemic IGF-I secretion in canine mammary tumours. PMID: 18367392
    13. IGF-1/IGF-1R and cyclins are involved in the hypertrophic response observed in cardiomyopathies. PMID: 19818143

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫鏈接：