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  4. Dog interleukin 4,IL-4 ELISA Kit

Dog interleukin 4,IL-4 ELISA Kit

  • 中文名稱：
    犬白介素4(IL-4)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E11259c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E11259c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL4含量。IL4是重要的細(xì)胞因子，在免疫應(yīng)答等生理過程發(fā)揮關(guān)鍵作用。其研究機(jī)制主要圍繞與IL4受體結(jié)合后激活下游信號(hào)通路，調(diào)節(jié)細(xì)胞增殖、分化等，在過敏、哮喘等疾病病理機(jī)制中有關(guān)鍵作用，也是藥物研發(fā)熱門靶點(diǎn)。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，適用于科研領(lǐng)域中對(duì)犬類免疫相關(guān)疾病機(jī)制的研究，例如過敏性疾病模型構(gòu)建、寄生蟲感染后免疫應(yīng)答分析、疫苗效果評(píng)估或免疫調(diào)節(jié)藥物開發(fā)等實(shí)驗(yàn)場(chǎng)景，為探索犬類免疫調(diào)控網(wǎng)絡(luò)提供精準(zhǔn)的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %87-97
    1:2Average %89
    Range %85-99
    1:4Average %98
    Range %92-105
    1:8Average %93
    Range %86-100
  • 回收率：
    The recovery of dog IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-105
    EDTA plasma (n=4)9285-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.912 2.986 2.949 2.853
    2002.546 2.531 2.539 2.443
    1001.780 1.831 1.806 1.710
    501.127 1.207 1.167 1.071
    250.546 0.588 0.567 0.471
    12.50.259 0.276 0.268 0.172
    6.250.168 0.176 0.172 0.076
    00.095 0.096 0.096 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 基因功能參考文獻(xiàn)：
    1. immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2, its receptor (IL-2R), IL-4 and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult and aged beagle dogs, were compared. PMID: 23605681
    2. In dogs with leishmaniasis, there was high expression of genes encoding IL-4 in blood leucocytes. The predominance of IL-4 gene expression in the blood of asymptomatic dogs may favour parasite replication. PMID: 21511273
    3. The anti-inflammatory and anabolic effects of regulated expression of IL-4 in chondrocyte-scaffolds under in vitro inflammatory conditions, were investigated. PMID: 21991344
    4. This study focuses on the co-expression of interleukin-4 (IL-4) and insulin-like-growth factor-1 (IGF-1), which specifically target inflammation and cartilage repair, respectively. PMID: 20188584
    5. These findings suggest that polyunsaturated fatty acids are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence cytokine transcription. PMID: 20187917
    6. IL-4 expressed in canine articular chondrocytes is biologically active and suppresses inflammatory mediators in vitro. PMID: 18789870

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: cfa:403785

    STRING: 9615.ENSCAFP00000001253

    UniGene: Cfa.39