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  4. Dog matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

Dog matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

  • 中文名稱：
    犬基質(zhì)金屬蛋白酶9/明膠酶B(MMP-9/Gelatinase B)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13887c
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬基質(zhì)金屬蛋白酶9/明膠酶B(MMP-9/Gelatinase B)酶聯(lián)免疫試劑盒（CSB-E13887c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的MMP9含量。MMP9 即基質(zhì)金屬蛋白酶 9，在細(xì)胞外基質(zhì)降解等過程中發(fā)揮重要作用。其異常表達(dá)與腫瘤侵襲轉(zhuǎn)移、炎癥反應(yīng)、心血管疾病等密切相關(guān)。研究機(jī)制涉及調(diào)控其表達(dá)與活性，探索相關(guān)信號通路，以開發(fā)針對 MMP9 的治療策略。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，的應(yīng)用場景包括但不限于犬類炎癥性疾病模型研究、腫瘤微環(huán)境分析、創(chuàng)傷愈合機(jī)制探索等基礎(chǔ)科研領(lǐng)域，為研究者提供可靠的蛋白表達(dá)數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    12.5 ng/mL-800 ng/mL
  • 靈敏度：
    3.12 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %99
    Range %92-105
    1:2Average %88
    Range %80-96
    1:4Average %87
    Range %85-90
    1:8Average %92
    Range %88-107
  • 回收率：
    The recovery of dog MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-89
    EDTA plasma (n=4)9790-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8002.096 2.076 2.086 1.977
    4001.513 1.605 1.559 1.450
    2001.135 1.118 1.127 1.018
    1000.618 0.655 0.637 0.528
    500.423 0.409 0.416 0.307
    250.301 0.278 0.290 0.181
    12.50.230 0.228 0.229 0.120
    00.110 0.107 0.109 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 數(shù)據(jù)庫鏈接：