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  4. Dog motilin (MLN) ELISA kit

Dog motilin (MLN) ELISA kit

  • 中文名稱：
    犬胃動素(MLN)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL014638DO
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬胃動素(MLN)酶聯(lián)免疫試劑盒（CSB-EL014638DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MLN含量。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，該產(chǎn)品適用于科研領(lǐng)域，例如在動物營養(yǎng)學(xué)實驗中評估飼料成分對胃腸激素分泌的影響，或在基礎(chǔ)醫(yī)學(xué)研究中探索胃腸功能紊亂的分子機制，為犬類消化生理及病理研究提供定量分析支持。研究犬消化系統(tǒng)疾病模型、胃腸動力調(diào)節(jié)機制或相關(guān)藥物干預(yù)實驗。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    MLN ELISA Kit; Motilin ELISA Kit
  • 縮寫：
    MLN
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog MLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-95  
    1:2 Average % 102  
    Range % 97-107  
    1:4 Average % 91  
    Range % 85-97  
    1:8 Average % 97  
    Range % 91-103  
  • 回收率：
    The recovery of dog MLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    8000 2.188 2.096 2.142 1.967  
    4000 1.487 1.406 1.447 1.272  
    2000 0.962 0.987 0.975 0.800  
    1000 0.626 0.607 0.617 0.442  
    500 0.434 0.468 0.451 0.276  
    250 0.361 0.335 0.348 0.173  
    125 0.292 0.281 0.287 0.112  
    0 0.171 0.178 0.175    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Motilin family
  • 數(shù)據(jù)庫鏈接：