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  4. Dog β2-microglobulin,BMG/β2-MG ELISA Kit

Dog β2-microglobulin,BMG/β2-MG ELISA Kit

  • 中文名稱：
    犬β2微球蛋白(BMG/β2-MG)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13768c
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    犬β2微球蛋白(BMG/β2-MG)酶聯(lián)免疫試劑盒（CSB-E13768c）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的B2M含量。B2M全稱為β2微球蛋白，其在免疫識(shí)別、抗原呈遞等過(guò)程中發(fā)揮重要作用。它與主要組織相容性復(fù)合體Ⅰ類分子結(jié)合，參與機(jī)體免疫監(jiān)視。研究發(fā)現(xiàn)其表達(dá)異常與腫瘤、自身免疫病等多種疾病相關(guān)，目前研究聚焦于其在疾病發(fā)生發(fā)展機(jī)制及作為治療靶點(diǎn)的潛力。試劑盒檢測(cè)范圍為0.78 μg/mL-50 μg/mL，適用于科研場(chǎng)景下犬類腎臟功能評(píng)估、免疫相關(guān)疾病機(jī)制研究或藥物毒性實(shí)驗(yàn)中生物標(biāo)志物的檢測(cè)，為動(dòng)物實(shí)驗(yàn)提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    B2M ELISA Kit; Beta-2-microglobulin ELISA Kit; Fragment ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.78 μg/mL-50 μg/mL
  • 靈敏度：
    0.2 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog β2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 83-99
    1:2 Average % 92
    Range % 85-100
    1:4 Average % 95
    Range % 85-105
    1:8 Average % 95
    Range % 88-102
  • 回收率：
    The recovery of dog β2-MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 86-99
    EDTA plasma (n=4) 95 91-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average Corrected
    50 2.554 2.569 2.562 2.430
    25 2.272 2.256 2.264 2.132
    12.5 1.792 1.803 1.798 1.666
    6.25 1.153 1.180 1.167 1.035
    3.13 0.625 0.616 0.621 0.489
    1.56 0.363 0.379 0.371 0.239
    0.78 0.261 0.264 0.263 0.131
    0 0.135 0.129 0.132 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫(kù)鏈接：

    UniGene: Cfa.29179