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  4. Fish (Salmon) leptin (LEP) ELISA kit

Fish (Salmon) leptin (LEP) ELISA kit

  • 中文名稱(chēng)：
    魚(yú)(鮭魚(yú))瘦素(LEP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL012870FI
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚(yú)(鮭魚(yú))瘦素(LEP)酶聯(lián)免疫試劑盒（CSB-EL012870FI）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的LEP含量。LEP即瘦素基因編碼蛋白瘦素，其在能量平衡和體重調(diào)控中扮演關(guān)鍵角色。研究機(jī)制主要是，瘦素能作用于下丘腦，通過(guò)信號(hào)傳導(dǎo)調(diào)節(jié)食欲、能量消耗等。當(dāng)下，圍繞LEP在治療肥胖、代謝性疾病等方面的研究不斷開(kāi)展，以發(fā)掘其更多治療潛力。試劑盒檢測(cè)范圍為62.5 pg/mL-1000 pg/mL，適用于魚(yú)類(lèi)生理學(xué)、營(yíng)養(yǎng)學(xué)及環(huán)境毒理學(xué)研究，可用于探究餌料成分、溫度變化或污染物暴露對(duì)魚(yú)類(lèi)代謝調(diào)控的影響，為水產(chǎn)養(yǎng)殖優(yōu)化和生態(tài)保護(hù)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Fish(Salmon)
  • 樣本類(lèi)型：
    serum, plasma
  • 檢測(cè)范圍：
    62.5 pg/mL-1000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 91  
    Range % 86-98  
    1:40 Average % 97  
    Range % 91-102  
    1:80 Average % 84  
    Range % 80-87  
    1:160 Average % 90  
    Range % 83-96  
  • 回收率：
    The recovery of fish LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-96  
    EDTA plasma (n=4) 97 93-102  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.082 0.086 0.084    
    500 0.190 0.200 0.195    
    250 0.483 0.444 0.464    
    125 0.945 0.959 0.952    
    62.5 1.672 1.555 1.614    
    0 2.591 2.670 2.631    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

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相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

https://www.cusabio.com/ELISA-Kit/Fish-Salmon-leptin-LEP-ELISA-kit-87315.html
Have you ever tested tissue sample?

A:
Thanks for your inquiry!We only tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test tissue samplet,we suggest do pre-testing first.Here is the processing way on tissue samples.Pls check for your reference:
Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was removed and assayed immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Q:

Is this kit also compatible to test Leptin levels in Zebrafish?

A:
Thanks for your inquiry!We suggest you do a pretest if you want to test zebrafish.
Q:

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please send the manual.
e. Please provide your reference data for fish plasma samples.

A:
Thanks for your inquiry.
CSB-EL012870FI
This is a competitive kit. There is only one antibody in the kit.
The plate is coated with mouse monoclonal antibody. ( Immunogen is CHO-derived recombinant protein.）
Standard is CHO-derived recombinant protein.
Pls find attached manual.
We tested salmon serum and plasma and some common freshwater fish before and the test value is about
125pg/ml under 1:100 dilution. The final concentration is about 12.5ng/ml.
Pls let me know if you have any further questions. Thank you.