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  4. Fish Acetylcholinesterase(AChE)ELISA Kit

Fish Acetylcholinesterase(AChE)ELISA Kit

  • 中文名稱：
    魚乙酰膽堿酯酶(AChE)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17001Fh
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚乙酰膽堿酯酶(AChE)酶聯(lián)免疫試劑盒（CSB-E17001Fh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的AChE含量。AChE即乙酰膽堿酯酶，是一種重要的神經(jīng)遞質(zhì)水解酶。背景上，它在神經(jīng)系統(tǒng)中對(duì)維持神經(jīng)沖動(dòng)正常傳導(dǎo)至關(guān)重要。研究機(jī)制方面，主要聚焦于其如何高效水解乙酰膽堿，以調(diào)控神經(jīng)信號(hào)傳遞，相關(guān)研究可為神經(jīng)系統(tǒng)疾病治療藥物研發(fā)提供方向。試劑盒檢測(cè)范圍為2.5 ng/mL-40 ng/mL，該產(chǎn)品適用于科研領(lǐng)域的環(huán)境毒理學(xué)研究，例如水體污染物對(duì)魚類神經(jīng)功能的影響評(píng)估、生態(tài)毒性機(jī)制探究或?qū)嶒?yàn)室條件下魚類模型的神經(jīng)生化指標(biāo)監(jiān)測(cè)，為水產(chǎn)養(yǎng)殖安全、環(huán)境污染生物標(biāo)志物篩選等研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    acheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    2.5 ng/mL-40 ng/mL
  • 靈敏度：
    2.5 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 94
    Range % 89-103
    1:2 Average % 92
    Range % 87-101
    1:4 Average % 93
    Range % 87-100
    1:8 Average % 89
    Range % 83-99
  • 回收率：
    The recovery of fish AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 84-93
    EDTA plasma (n=4) 94 87-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average 　
    40 0.284 0.292 0.288 　
    20 0.521 0.532 0.527 　
    10 0.886 0.891 0.889 　
    5 1.374 1.382 1.378 　
    2.5 2.583 2.599 2.591 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
  • 亞細(xì)胞定位：
    [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.; [Isoform T]: Cell membrane; Peripheral membrane protein. Cell junction, synapse. Note=Attached to the membrane through disulfide linkage with the collagenic subunit, itself bound to the membrane.
  • 蛋白家族：
    Type-B carboxylesterase/lipase family
  • 組織特異性：
    Found in the synapses and to a lower extent in extrajunctional areas of muscle and nerve, and on erythrocyte membranes.