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  4. Fish Aromatase(ARO) ELISA Kit

Fish Aromatase(ARO) ELISA Kit

  • 中文名稱：
    魚芳香酶酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL006394FI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚芳香酶酶聯(lián)免疫試劑盒（CSB-EL006394FI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYP19A1含量。CYP19A1是一種細胞色素P450酶，也叫芳香化酶，主要負責(zé)催化雄激素向雌激素轉(zhuǎn)化。其表達異常與乳腺癌等激素依賴性疾病的發(fā)生發(fā)展密切相關(guān)，目前是治療此類疾病的重要靶點，研究機制多圍繞抑制其活性以降低雌激素水平展開。試劑盒檢測范圍為25 pg/mL-1000 pg/mL，適用于魚類內(nèi)分泌調(diào)節(jié)機制研究、生殖生理學(xué)實驗及環(huán)境激素干擾評估，例如通過檢測血清或組織勻漿中的芳香酶水平，探究污染物對魚類性腺發(fā)育的影響，或在水產(chǎn)養(yǎng)殖中分析激素代謝與繁殖性能的關(guān)聯(lián)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    cyp19a1 ELISA kit; cyp19Aromatase ELISA kit; EC 1.14.14.14 ELISA kit; CYPXIX ELISA kit; Cytochrome P-450AROM ELISA kit; Cytochrome P450 19A1 ELISA kit; Estrogen synthase ELISA kit
  • 縮寫：
    CYP19A1
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    25 pg/mL-1000 pg/mL
  • 靈敏度：
    25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish ARO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 89-104    
    1:2 Average % 95    
    Range % 86-100    
    1:4 Average % 95    
    Range % 87-106    
    1:8 Average % 89    
    Range % 86-99    
  • 回收率：
    The recovery of fish ARO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 89-103    
    EDTA plasma (n=4) 94 90-100    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average 　    
    1000 0.154 0.156 0.155 　    
    500 0.359 0.355 0.357 　    
    230 0.573 0.564 0.569 　    
    75 0.877 0.862 0.870 　    
    25 1.561 1.583 1.572 　    
    0 2.342 2.369 2.356 　    
  • 本試劑盒所含材料：
    • A 96-well Assay plate--The 96-well plate has been pre-coated with goat-anti-rabbit ARO antibody.
    • Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • HRP-conjugated ARO (1 x 6 ml) --Bind to the anti-fish ARO antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • Anti-fish ARO Antibody (1 x 6 ml)--React with both ARO in the samples/standards and HRP-conjugated ARO.
    • Wash Buffer (20x concentrate) (1 x 15ml) --Wash away unbound or free substances.
    • Substrate A (1 x 7 ml) --Mix with Substrate B and interacts with HRP, eliciting the solution turns blue.
    • Substrate B (1 x 7 ml) --Mix with Substrate A and interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

Per customers request, please provide the following information regarding Fish Aromatase, ARO ELISA Kit CSB-EL006394FI
1) What sample types have been tested with this kit.
2) What observed concentrations were obtained from these sample types and what dilutions were used
3) Please provide the QC data for sample tested with this kit.
4) Is the capture antibody a monoclonal or polyclonal?
5) What is the host of the capture antibody?
6) What’s the immunogen or immunogen range of the capture antibody?
7) What’s is the species of the immunogen of the capture antibody, human or something else?
8) Is the detection antibody a monoclonal or polyclonal?
9) What is the host of the detection antibody?
10) What is the immunogen or immunogen range of the detection antibody?
11) What is the species of the immunogen of the detection antibody? Human or something else?
12) What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
13) If recombinant protein, is it full length or partial?
14) What is the species of the standard– human or something else?

A:
Thanks for your inquiry!
CSB-EL006394FI
This is a competitive kit and there is only one antibody in the kit.
The plate is coated with rabbit polyclonal antibody.
Immunogen is 293 cell-derived recombinant protein. It is affinity purified.
Standard is 293 cell-derived recombinant protein.
This kit is designed according to: https://www.uniprot.org/uniprot/Q92087.
We tested undiluted serum and plasma samples before and the test value is 25--75pg/ml.
Pls find attached QC.
Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 功能：
    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
  • 亞細胞定位：
    Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 數(shù)據(jù)庫鏈接：