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Fish Catalase (CAT) ELISA kit

  • 中文名稱:
    魚過氧化氫酶(CAT)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E15928Fh
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    魚過氧化氫酶(CAT)酶聯(lián)免疫試劑盒(CSB-E15928Fh)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的CAT含量。試劑盒檢測范圍為125 mU/mL-2000 mU/mL,該產(chǎn)品可應(yīng)用于水產(chǎn)養(yǎng)殖研究中魚類健康狀態(tài)監(jiān)測、水體污染對生物抗氧化系統(tǒng)的影響評估,以及環(huán)境毒理學(xué)中重金屬、有機(jī)污染物等脅迫因子的效應(yīng)機(jī)制研究,為魚類生理生化研究提供可靠的實驗工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Fish
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    125 mU/mL-2000 mU/mL
  • 靈敏度:
    62.5 mU/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 91-99  
    1:200 Average % 103  
    Range % 99-107  
    1:400 Average % 98  
    Range % 93-105  
    1:800 Average % 92  
    Range % 87-95  
  • 回收率:
    The recovery of fish CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-104  
    EDTA plasma (n=4) 88 83-96  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average    
    2000 0.091 0.089 0.090    
    1000 0.242 0.257 0.250    
    500 0.553 0.587 0.570    
    250 0.913 0.980 0.947    
    125 1.647 1.563 1.605    
    0 2.381 2.319 2.350    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

靶點詳情

  • 最新研究進(jìn)展:
    CAT是一種重要的抗氧化酶,在機(jī)體中起到清除自由基的作用,對細(xì)胞和組織的損傷具有保護(hù)作用。最新研究表明,CAT在代謝性疾病、腫瘤和神經(jīng)系統(tǒng)等多個疾病的發(fā)生和發(fā)展中發(fā)揮著重要作用,通過抑制細(xì)胞凋亡、調(diào)節(jié)免疫反應(yīng)等多種機(jī)制發(fā)揮保護(hù)作用,成為疾病治療的潛在靶點。