Fish Cholecystokinin(CCK) ELISA Kit

產(chǎn)品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

魚膽囊收縮素/腸促胰酶肽(CCK)酶聯(lián)免疫試劑盒（CSB-E13054Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCK含量。CCK（膽囊收縮素）是一種胃腸道及神經(jīng)系統(tǒng)分泌的激素，主要作用是刺激膽囊收縮和促進胰腺分泌。其研究機制涉及通過靶細(xì)胞表面的受體發(fā)揮作用，調(diào)節(jié)胃腸運動、食欲調(diào)節(jié)、激素分泌等生理過程。CCK在消化系統(tǒng)和神經(jīng)系統(tǒng)中的作用密切相關(guān)，通過腦腸軸實現(xiàn)雙向調(diào)節(jié)，影響多種生理和病理過程。試劑盒檢測范圍為200 pg/mL-5000 pg/mL，本試劑盒適用于實驗室環(huán)境下的樣本檢測，可應(yīng)用于水產(chǎn)養(yǎng)殖研究、魚類生理學(xué)實驗或環(huán)境毒理學(xué)評估等場景，為水生生物基礎(chǔ)研究提供高靈敏度的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CCK
Uniprot No.：

D7S9W7
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

200 pg/mL-5000 pg/mL
靈敏度：

125 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-105
1:2	Average %	89
	Range %	84-96
1:4	Average %	96
	Range %	91-102
1:8	Average %	93
	Range %	86-105

回收率：

The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-91
EDTA plasma (n=4)	95	86-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.