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  4. Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

  • 中文名稱：
    魚細胞色素P450 1A1(CYP1A1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL006395FI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    魚細胞色素P450 1A1(CYP1A1)酶聯(lián)免疫試劑盒（CSB-EL006395FI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYP1A1含量。CYP1A1是一種主要的細胞色素P450酶，廣泛研究其激活致癌化合物的能力。它通過芳香烴受體（AhR）介導的轉(zhuǎn)錄激活，在代謝環(huán)境致癌物和膳食化合物中發(fā)揮重要作用。CYP1A1在肺癌等癌癥的發(fā)生發(fā)展中扮演關鍵角色，其研究機制涉及致癌物激活、基因多態(tài)性、以及與AhR等受體的相互作用。試劑盒檢測范圍為75 pg/mL-1200 pg/mL，適用于科研場景中評估魚類暴露于環(huán)境毒素后的生理響應、污染物生物有效性研究以及生態(tài)毒理學模型的建立。為水生生態(tài)系統(tǒng)健康監(jiān)測、污染物代謝機制研究及水產(chǎn)養(yǎng)殖安全評估提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    cyp1a1 ELISA kit; cyp1aCytochrome P450 1A1 ELISA kit; EC 1.14.14.1 ELISA kit; CYPIA1 ELISA kit
  • 縮寫：
    CYP1A1
  • Uniprot No.：
  • 種屬：
    Fish
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    75 pg/mL-1200 pg/mL
  • 靈敏度：
    75 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish CYP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-98  
    1:2 Average % 100  
    Range % 92-107  
    1:4 Average % 85  
    Range % 80-91  
    1:8 Average % 88  
    Range % 83-96  
  • 回收率：
    The recovery of fish CYP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 88-95  
    EDTA plasma (n=4) 94 91-97  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1200 0.146 0.147 0.147    
    600 0.238 0.254 0.246    
    300 0.524 0.553 0.539    
    150 1.112 1.135 1.124    
    75 2.338 2.277 2.308    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • 亞細胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 數(shù)據(jù)庫鏈接：